二硫化碳作业工人尿中皮质激素及代谢产物的检测及生物监测价值初探

Preliminary Studies on Detection of Corticosteroid Hormones and Their Metabolites in Urine and Blood of Workers Exposed to Carbon Disulfide and Its Application in Biological Monitoring

为了解二硫化碳（ＣＳ２）对肾上腺皮质功能的影响，探讨其作为ＣＳ２暴露生物监测指标的可行性及价值，采用ＨＰＬＣ法检测尿中皮质激素水平，并调查一组长期接触ＣＳ２的化纤生产工人血、尿中皮质激素及代谢产物的变化。结果表明接触超标浓度ＣＳ２的工人出现血、尿液皮质激素及代谢产物的减少。多元逐步回归分析表明肾上腺皮质激素的改变与空气ＣＳ２浓度密切相关。尿中皮质激素（游离皮质醇、可的松和醛固酮）以及代谢产物１７－酮类固醇可望成为反映肾上腺皮质功能状况的无损伤性生物监测指标。

In order to study the effects of carbon disulfide(CS2)on the function of adrenal glands and the feasibility of its application in biological monitoring,urine and blood levels and corticosteroid hormones and their metabolites and their changes were detected with high performance liquid chromatography and radioimmunoassay for the workers exposed to CS2 for long term in a synthetic fiber works.Results showed that blood and urine levels of corticosteroid hormones and their metabolites declined in the workers exposed to CS2 at the air concentrations in the workplace exceeding the national hygienic standard.Analysis with multiple stepwise regression revealed that changes in adrenal corticosteroid hormones correlated with CS2 concentrations in workplace air.It suggests that urine levels of corticosteroid hormones,including free cortisol,cortisone and aldosterone which reflect the functional status of adrenal glands,and their metabolites can be used as indicators for nontraumatic effects in biological monitoring..