白花蛇舌草提取液诱导多药耐药白血病细胞HL-60/ADR凋亡的研究

Study the Inhibitory Action and Inducing Apoptosis of Acetic acid ethyl ester Extracts from Hedyotic diffusa on Multidrug Resistance Leukemic cell line HL-60/ADR

目的:研究白花蛇舌草乙酸乙酯提取液对多药耐药白血病细胞HL-60/ADR的抑制作用及其机制。方法:采用MTT比色试验观察白花蛇舌草乙酸乙酯提取液对多药耐药细胞HL-60/ADR的抑制作用(HL-60/ADR的耐药倍数为204倍)。采用光镜、电镜技术观察细胞形态结构的改变,利用流式细胞术(FCM)检测细胞凋亡率。结果:白花蛇舌草乙酸乙酯提取液能明显抑制HL-60/ADR细胞的生长,药物浓度相当于生药41.67mg/ml时,即具有显著的抑制作用,抑制率呈剂量和时间依赖性,半数抑制浓度(IC50)相当于生药70.41mg/ml。流式细胞学证实白花蛇舌草乙酸乙酯提取液,能诱导多药耐药细胞HL-60/ADR凋亡,凋亡率也呈时间和剂量的依赖性。结论:白花蛇舌草对多药耐药细胞HL-60/ADR的生长具有极强的抑制作用,诱导其凋亡是其机制之一。

Objective ： To study the inhibitory action and mechanism of acetic acid ethyl ester extracts from Hedyotic diffusa on muhidrug resistance leukemic cell line HL-60/ADR. Methods ： To observe the depressant effect of acetic acid ethyl ester extracts from Hedyotic diffusa on muhidrug resistance leukemic cell line HL-60/ADR （the drag-fast multiple of HL-60/ADR is 204）by MTF. To observe the change of the cells morphology structure by light microscope and electron microscope, to detect the apoptosis rate by flow cytometry （FCM）. Results ： The extracts from Hedyotic diffusa could effectively inhibit the proliferation of HL-60/ADRcells. When the concentration is amount to 41.67mg/ml in pharmacognostics, it has effectively inhibitory action. The inhibition rate has dependence of dosage and time. The IC50 is amount to 70.41 mg/ml in pharmacognostics. Acetic acid ethyl ester extracts from Hedyotic diffusa could induce apoptosis of muhidrug resistance leukemic cell line HL-60/ADR have been confirmed by flow cytometry（FCM） ,the apoptotic rate has dependence of dosage and time. Conclusion： Hedyotic diffusa could effectively inhibit the proliferation of muhidrug resistance leukemic cell line HL-60/ADR,induce HL-60/ADR to apoptosis is one of its mechanism.