叶下珠荧光物质的分离和性质研究

Separation and properties of fluorescent compound in phyllantuus urinaria L.

目的:研究叶下珠药材中的荧光活性物质及性质,为光敏剂的筛选提供资料。方法:采用50%乙醇提取、乙酸乙酯萃取,对叶下珠植物提取分离。采用荧光扫描确定提取液激发波长和发射波长。提取液经硅胶柱层析,氯仿:甲醇:磷酸(85:14:1)洗脱。结果:提取液最小激发波长和最大发射波长分别为418nm和667nm。硅胶柱层析获得6种组分,其中一种组分,经418nm光激发后,在667nm处具有较强的荧光发射峰。pH酸性、提高温度和光照均可提高其荧光强度,碱性条件会导致其荧光猝灭。通过液质测定,推断其为珠子草素。结论:该成分具有光敏剂开发前景。

Objective：To research the fluorescent compound in the plant of Phyllantuus urinaria L and its properties, provide the data for screening of photo sensitizer. Methods： The plant was extracted by 50% ethanol, ethyl acetate, then purified hy silica gel column with the elution of chloroform： methanol： phosphoric acid （85：14：1）. The excitation and emission wavelength were seanned by fluorescence spectrophotometer. Results： The minexcitation and maxenmission wavelength of extract were respectively 418nm and 667run. Six fractions were obtained from the elution. One compound separated by silica gel column was found to have a stronger fluorescence emission at 667nm by excitation of laser at 418nm. Acidity, heating and illumination in a certain degree increased its fluorescence intensity,but alkalinity caused fluorescence quenching. It was speculated as niranthin by HPLC/MS. Conclusion： It was a new potential photo sensitizer.