三黄汤煎剂中黄芩苷在大鼠体内药动学研究

Pharmacokinetics of Baicalin of Sanhuang Decoction in Rats

目的:研究三黄汤煎剂中黄苓苷在大鼠体内的药动学规律。方法:大鼠ig三黄汤煎剂后,在规定时间取血,用高效液相色谱法测定血浆中黄芩苷浓度,并计算主要药动学参数结果:大鼠体内黄芩苷的主要药动学参数分别为t_(max1)=(15±4.23)min, t_(max2)=(6.8±0.54)h,C_(max1)=(4.49±1.56)μg·mL^(-1),C_(max2)=(3.25±1.23)μg·mL^(-1),AUC(0-18)=(35.52±12.35)μg·h·mL^(-1),t_(1-2)=(5.12±0.23)h,CL=(3.86±0.91)L·h^(-1)结论:该方法样品处理简单,快速准确,专属性好,灵敏度较高,可作为含黄芩苷中成药的血药浓度监测手段。

OBJECTIVE： To investigate the pharmacokinetic profiles of baicalin （a constituent in Sanhuang decoction） in rats. METHODS： Blood samples were collected as schedule from rats after intragastrically administration of Sanhuang decoction, then the content of baicalin was determined by HPLC and the chief pharmacokinetic parameters were computed. RESULTS： The chief pharmacokinetic parameters of haicalin in rats were as follows： tmax1=（15±4.23）min.tmax2=（6.8±0.54）h,Cmax1=（4.49±1.56）μg·mL^-1,Cmax2=（3.25±1.23）μg·mL^-1,AUC0-18=（35.52±12.35）μg·h·mL^-1,t1/2=（5.12±0.23）h,CL=（3.86±0.91）L·h^-1. CONCLUSION： The method is simple in sample treatment, rapid and accurate, specific and sensitive, and it can be used as a means for the monitoring of the serum concentration of baicalin containing Chinese patent medicines and so as to provide reference for clinical rational drug use.