HPLC法测定新温肾消肿丸中氢氯噻嗪的含量

Determination of Hydrochlorothiazide in New Wenshen Xiaozhong Pill by HPLC

目的:建立以高效液相色谱法测定新温肾消肿丸中氢氯噻嗪含量的方法方法:色谱柱为Kromasil 60-5CN柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.05 moL·L^(-1)磷酸二氢钾溶液(磷酸调pH 3.0)=49:51,检测波长为226 nm。结果:氢氯噻嗪的检测浓度在2.227～44.540μg·mL^(-1)范围内与峰面积积分值呈良好线性关系(r=0.9999);平均回收率为100.70%, RSD=1.64%(n=6)。结论:本法简便、快速、准确,可用于新温肾消肿丸的质量控制。

OBJECTIVE： To develop an HPLC method for the determination of hydrochlorothiazide in New wenshen xiaozhong pill. METHODS： The determination was performed on a Kromasil 60- 5CN（250 mm × 4.6 mm, 5 μm） .The mobile phase consisted of methanol-0.05 mol·L^-1 potassium dihydrogen phosphate （pH = 3.0 adjusted by phosphoric） （49 ： 51） . The UV detection wavelength was set at 226 nm. RESULTS： The linear range of hydrochlorothiazide was 2.227--44.540μg·mL^-1（r = 0.999 9） with an average recovery rate of 100.70% （RSD = 1.64%, n = 6） .CONCLUSION： The method is simple, rapid, accurate and applicable for the quality control of New wenshen xiaozhong pill.