摘要
应用Fura-2荧光技术和原子吸收分光光度法测定大鼠心肌内钙，结果发现；对照组、缺血组和治疗组大鼠心肌细胞内游离钙（MyoCai）分别为139．5±7.4、525．9±106．7和202．2±53.5nmol／L；对照组和缺血组大鼠心肌细胞总钙（MyoCat）分别为119.0±17．5和135．8±26.5mmol／kg·干重；MyoCai和MyoCat间无相关性。研究表明：缺血早期MyoCai明显增高，但MyoCat变化不明显；尼莫地平能阻止缺血心肌细胞钙超载的发展和加重。
The concentrations of myocyte free calcium and the content of myocyte total calcium in rat were measured by Ca indicator Fura-2 and atomic adsorption spectrophotometry, respectively. The results showed that (1) the concentration of myocyce free calcium in control rats, ischemic rats and treated rats were 139.5±7.4,525.9±106.7, and 202.2±53.5nmol/L, respectively; (2) the contents of myocyte total calcium in central rats,ischemic rats were 119.0±17.5 and 135.8±26.5 mmol/Kg dry weight, respectively; (3) there was no correlation between myocyte free calcium and total calcium. The results indicated that the concentration of myocyte free calcium in ischemia increased significantly, but there was no obvious change in the content of myocyte total calcium in the early stage of ischemia. Nimodipine could prevent the calcium overload in ischemic myocyte.
出处
《心肺血管病杂志》
CAS
1998年第1期45-46,共2页
Journal of Cardiovascular and Pulmonary Diseases
基金
国家自然科学基金!39370716
