介孔纳米SiO_2致小鼠巨噬细胞株RAW264.7细胞毒性与氧化损伤

In vitro Cytotoxicity and Oxidative Damage of Mesoporous Nano-SiO_2 on RAW264.7 Macrophages

[目的]探讨介孔纳米SiO2对小鼠巨噬细胞株RAW264.7细胞的毒性和氧化损伤作用。[方法]设4个浓度组(2.5、10、50、100μg/mL)和生理盐水对照组,染毒24h后,用四甲基偶氮唑盐(MTT)方法观察细胞毒性,并根据染毒后细胞的总蛋白(TP)、乳酸脱氢酶(LDH)、一氧化氮(NO)、谷胱甘肽(GSH)、超氧化物歧化酶(SOD)和丙二醛(MDA)的变化情况分析介孔纳米SiO2的细胞毒性和氧化损伤作用。[结果]10、50和100μg/mL浓度组细胞存活率随着染毒浓度的升高而降低,差异有统计学意义;50、100μg/mL浓度组细胞及其上清液中TP、LDH、NO和MDA含量随染毒浓度增加而升高,而GSH和SOD含量降低;而且与细胞发生融解破碎、间隙加大等形态学改变情况相符。[结论]介孔纳米SiO2对体外培养巨噬细胞株RAW264.7具有细胞毒性和氧化损伤作用,且随剂量的加大而增强。

[Objective] To study the in vitro cytotoxicity and oxidative damage of mesoporous nano-Si02 on RAW264.7 macrophages, [ Methods ] The RAW 264.7 cells were treated with different concentrations of mesoporous nano-Si02（ 2.5, 10, 50, 100μg/mL ）and saline control for 24h. MrIT assays were applied for testing macrophage cytotoxicity in vitro, which were based on mitochondrial activity evaluation. Total protein （TP）, lactate dehydrogenase （LDH）, nitrogen monoxide （NO）, glutathion （ GSH ）, superoxide dismutase （ SOD ） and malondialdehyde （ MDA ） in supernatant and intra-cellular fluid were determined. The morphologic change of RAW 264.7 was also observed. [ Results ] Compared with saline control, the viability of RAW264.7 cell was decreased significantly after exposed to mesoporous nano-SiO2. The levels of TP, LDH, NO and MDA in exposed groups of 50μg/mL and 100μg/mL dosage were higher, while GSH and SOD levels were lower than in the saline control group, which were in accordance with the morphologic change on RAW264.7 cell exposed to mesoporous nano-SiO2. [ Conclusion ] Mesoporous nano- Si02 can induce cytotoxicity and oxidative demage on RAW264.7 macrophage.