血小板激活因子拮抗剂BN52021对缺血再灌注损伤的大鼠肝脏的保护作用

Protection of BN 52021 on Liver IschemiaReperfusion Injuries in Rats

目的：研究血小板激活因子拮抗剂ＢＮ５２０２１对大鼠缺血再灌注肝脏的影响。方法：将大鼠分为三组，对照组未进行缺血及药物治疗；缺血再灌注组行４０ｍｉｎ缺血，然后行１２０ｍｉｎ再灌注；血小板激活因子拮抗剂治疗组行４０ｍｉｎ缺血，１２０ｍｉｎ再灌注，再灌注时给予ＢＮ５２０２１（１０ｍｇ／ｋｇ），比较各组血清ＳＧＰＴ、ＳＧＯＴ、ＡＫＰ及γ－ＧＴ，肝脏的细胞能荷。结果：用血小板激活因子拮抗剂治疗组血清ＳＧＰＴ、ＳＧＯＴ、ＡＫＰ、γＧＴ均较缺血再灌注组显著降低，能荷水平明显升高。结论：血小板激活因子拮抗剂ＢＮ５２０２１用于大鼠肝脏缺血再灌注可减少肝脏的损害，提示血小板激活因子（ＰＡＦ）对肝脏缺血再灌注损伤的发生起一定的作用，血小板激活因子拮抗剂ＢＮ５２０２１有可能用于肝脏缺血再灌注损伤的治疗。

Objective: Plateletactivating factor antagonist BN 52021 on ischemia-reperfusion(I/R)injury of rat liver was studied.Methods: Rats were divided into 3 groups.The normal control group was not subjected to hepatic ischemia and no treatment was given.The I/R injury group was subjected to 40 minutes of ischemia followed by reperfusion for 120 minutes.The AntiPAF treatment group was subjected of reperfusion and BN 52021 10 mg/kg was administered at reperfusion SGPT,SGOT,AKP and γGT and hepatic energy charge were compared.Results: The antiPAF treatment group serum levels of SGPT,SGOT,AKP and γGT were significantly lower than I/R injury group. Energy charge level was significantly higher with antiPAF treatment (P<0.05).Conclusion: AntiPAF BN 52021 treatment at reperfusion decreased damage of rat liver subjected to I/R injury.AntiPAF BN 52021 may be useful in the treatment of hepatic I/R injury.