c-myc癌基因蛋白在喉癌及癌旁不同距离组织中的表达研究

A Study of the Expression of cmyc Oncogen Protein in Laryngeal Cancer and Different Distant Mucosa Adjacent to Cancer

目的：研究ｃｍｙｃ癌基因在喉癌及癌旁不同距离组织中的表达特点及意义。方法：应用枸橼酸－ＬＳＡＢ－微波免疫组化法，检测了ｃｍｙｃ癌基因蛋白在３０例喉癌边缘区及癌旁０５、１０、１５、２０ｃｍ处粘膜和４例正常喉粘膜中的表达。结果：ｃｍｙｃ蛋白在喉癌组织中的阳性表达率为９６７％（２９／３０），与正常组织相比差异显著（Ｐ＜００１），在癌旁１５ｃｍ处１０ｃｍ以内组织的表达存在着显著性差异（Ｐ＜００５）。ｃｍｙｃ表达从正常粘膜→癌旁组织→癌呈逐步递增趋势。结论：ｃｍｙｃ癌基因过度表达与喉癌的发生有关，且在喉癌发生中可能为一早期事件，距肿瘤１０ｃｍ以内之癌旁组织应视为危险病变。

Objective: In order to study occurrence and significant of cmyc expression in laryngeal carcinoma and different distant mucosa. Methods: Using citric-LSAB-microwave immunohistochemical technique,the expression of cmyc was studied in different sifes:30 cases of laryngeal squamous cell carcinoma (LSCC),border area.Adjacent mucosa which was 0.5 cm、1.0 cm、1.5 cm、2.0 cm away from cancer and four cases normal laryngeal mucosa.Results: Over-expression of cmyc in LSCC was found in 29 of 30 (96.7%),which was higher than that in normal laryngeal mucosa (P<0.01),significant different expression of cmyc can be seen at places 1.5 cm and 1.0 cm distant from the tumor (P<0.05).The expression of cmyc increased as tissue progressed in sequence of normal mucosa,adjacent mucosa and carcinoma.The cmyc expression did not correlate with site of tumor,stage and histological grade (P>0.05).Conclusion: Over-expression of cmyc is involved in genesis of LSCC,and may be an early event in the development of human squamous-cell carcinoma of the larynx.The tissue 1.0 cm adjacent to the tumor should be regarded as dangerous lesion.