IGF-1及TNF-α对大鼠成骨肉瘤细胞增殖的调节作用

The Effect of IGF1 and TNFα on the Regulator of Cell Proliforation in Osteoblist-like Cells

目的：通过对大鼠成骨肉瘤ＵＭＲ１０６细胞增殖状态及细胞周期变化的观察，探讨胰岛素样生长因子１（ＩＧＦ１）及肿瘤坏死因子α（ＴＮＦα）对该细胞增殖调节作用的机理。方法：将ＵＭＲ１０６细胞培养于含１０％小牛血清的ＭＥＭ培养基中。细胞长满后换无血清培养７２ｈ，然后分别用生理剂量的ＩＧＦ１和ＴＮＦα刺激细胞１２ｈ，应用３ＨＴｄＲ参入、磺基罗丹明染色法和流式细胞技术对细胞增殖状态及细胞周期进行定量测定。结果：ＩＧＦ１有明显促细胞ＤＮＡ合成作用，并使Ｓ期细胞所占比例明显增加；而ＴＮＦα则具有相反的作用。定量测定细胞增殖也显示ＵＭＲ１０６受ＩＧＦ１的正性调节而受ＴＮＦα的负性调节。结论：ＩＧＦ１和ＴＮＦα对ＵＭＲ１０６细胞增殖的调节作用与该细胞ＤＮＡ复制水平调节有关。

Objective: In this experiment rat osteoblastsarcoma cell line UMR 106,was used to study the effects of insulin-like growth factor 1 (IGF-1) and tumor necrosis factor (TNF-α) on the regulation of cell proliferation.Methods: UMR 106 cells were grown in MEM supplemented with 10% (v/v)FBS.After 48 h the cells were refed with serum-free medium.In there they were cultured for 72 h and treated with growth factor for 12 h. DNA synthesis was measured by using3HTdR incorporation.The cell number was measured by the SRB staining and the DNA cell-cycle analysis was measured by the flow cytometry(FCM).Results: The cells were treated with IGF-1(10-8mol/L).It was revealed that 3HTdR incorporation increased by 93.57% respectively,and the percentage of cells in S stage increased from 48.20% to 66.0%.In the case of TNF-α(10-8 mol/L) 3HTdR incorporation decreased by 57.51%,and the percentage of cells in S stage decreased from 49.30% to 22.60%.At the meantime,cell count were also increased in the definite period after IGF1 treatment and decreased after TNFα treatment.Conclusion: In conclusion,these two kinds of growth factor are the potent effector on the cell proliferition in UMR 106.