摘要
目的分析ICR小鼠线粒体ATPase6和D—Loop基因多态性及封闭群ICR小鼠遗传稳定性。方法在一个亲本雌鼠100只繁殖产生的共1000只后代群体中随机取12周龄雄鼠20只，提取小鼠睾丸生殖细胞DNA，PCR扩增小鼠线粒体ATPase6和D-Loop基因，产物纯化后测序，测序结果与小鼠线粒体标准序列比对，比较20只小鼠的序列差异。结果检测的20只小鼠ATPase6和D—Loop基因序列完全一致，且与小鼠线粒体标准序列完全一致。结论ICR小鼠群体内未检测到ATPase6和D—Loop基因的多态性变异，该群体具有较好的线粒体遗传稳定性。
Objective To analyse genetic polymorphisms and genetic stability in mitochondrial ATPase6 and D-Loop gene of Closed Colony mice. Methods Twenty male ICR mice from 1000 mice of stochastic colony （offspring of 100 female parent mice） were studied, mitochondria ATPase6 gene and D-Loop gene of them were amplified respectively by using PCR of two pairs primers. The results from DNA sequencing were compared with the standard sequence of mice. Results The sequence of mtATPase6 gene and D-Loop gene was same in twenty ICR mice. Conclusion Polymorphismof mitochondrial DNA was not found. The ICR mice colony showed good genetic stability.
出处
《实验动物与比较医学》
CAS
2009年第2期113-116,共4页
Laboratory Animal and Comparative Medicine
基金
温州医学院发展基金XNK04050
