摘要
以欧洲李（Prunus domestica cv．‘Tardicots’）和樱桃李（Prunus cerasifera）试管苗为材料，建立了茎尖离体培养与植株再生体系。结果表明：欧洲李最适宜的培养基为MS＋6-BA0．5mg／L＋IBA0．05mg／L，0．1～0．2mm的茎尖培养成苗率为64．16％，0．35～0．45mm的茎尖培养成苗率为85．5％；樱桃李最适宜的培养基为LP＋6-BA0．5mg／L＋IBA0．05mg／L，0．1～0．2mm的茎尖培养成苗率为64．53％，0．35～0．45mm的茎尖培养成苗率为84．7％。
Europe plurn（Prunus domestica cv. ＇Tardicots＇） and myrobalan（P, cerasifera） were used as the materials and the regeneration system was established using the shoot-tips as explants. The results showed that the optimal medium for Europe plum shoot-tips culture was MS ＋6-BA 0. 50 mg/L＋IBA 0. 05 mg/L,by 64. 16% and 85. 5% of seedling rate with 0. 1-0. 2 mm of shoot-tips and 0. 35-0. 45 mm of shoot-tips respectively. The optimal medium for myrobalan shoot-tips culture was LP ＋6-BA 0. 5 mg/L＋IBA 0. 05 mg/L, by 64. 53% and 84. 7% of seedling rate with 0. 1-0. 2 mm of shoot-tips and 0. 35-0. 45 mm of shoot-tips respectively.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2009年第2期214-217,共4页
Journal of Huazhong Agricultural University
基金
湖北省重点科技攻关项目(2001AA209B-140)资助
关键词
李
茎尖
离体培养
plum
shoot-tips
in vitro culture
