摘要
目的:探索连续30天对大白鼠腹腔注射氢溴酸东莨菪碱SCOP 3mg/kg能否作为老年性痴呆的造模方法,并探讨浅刺调督法对老年性痴呆的作用机制。方法:模型组、预防组及治疗组采用氢溴酸东莨菪碱腹腔注射3mg/kg,空白组予等量生理盐水腹腔注射,每日1次,连续30天,预防组在实验之日起针刺,治疗组在造模30天后,进行针刺治疗,连续30天。再采用Morris水迷宫检测各组大白鼠的学习与记忆行为。各组实验结束后,抽取大鼠腹腔静脉血5mL,完成后立即快速断头取脑,在冰盘上分离大鼠海马组织,保存在-75℃冰箱内,待进行生化测定。结果:海马胆碱酯酶(CHE)及血液中丙二醛(MDA)检测指标模型组与空白组有显著差别,说明了浅刺调督法对老年性痴呆的部分作用机制,及浅刺调督法对老年性痴呆模型有一定的作用效果。
Objective: To explore how to make Alzheimer' s disease model and what is its mechanism, treated by shallow acupuncture to regulate the Governor. Methods: Rats were divided into four groups: the control group, model group, prevention group and treated group. The model group, prevention group and treated group were injected scopolamine hydrobromide injection 3mg/kg at abdomen, 0.9% sodium chloride injection for the control group, once a day, successive 30 days. The prevention group was acupunctured from the first day, once a day, successive 30 days , and the treated group was treated after 30 days , method was the same with the prevention group. Then tested the rats'memory and activity by Morris water - maze. At the end of the experiment , taken out blood from inferior vena cava 5ml and hippocampus for every rat , saved in - 75℃ refrigeratory for test. Results: Compared with the control group, the model group showed an significant difference on the levels of Hippocampus Cholinesterase and Maleic Dialdehyde of blood . It told us that the shallow acupuncture by regulating the Governor can treat Alzheimer' s disease, and illustrate what is its mechanism.
出处
《辽宁中医杂志》
CAS
北大核心
2008年第12期1931-1933,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
广西自然基金资助项目(桂科自0542098)
关键词
浅刺调督法
氢溴酸东莨菪碱注射液
海马CHE
血液MDA
Regulate the Governor with the shallow acupuncture
Scopolamine hydrobromide injection
Hippocampus CHE
Blood MDA