诃子制草乌对蒙药嘎日迪-5镇痛抗炎作用的影响

Effect of Myrobalan System Wild Aconite on Mongolian Medicine Enlightens-5 Anti-inflammatory and Analgesic Function

目的:不同条件诃子制草乌对蒙药嘎日迪-5镇痛抗炎作用的影响。方法:比较3个不同条件(诃子汤中浸泡12h,煎煮2h[A]、诃子汤中浸泡24h,煎煮2h[B]、诃子汤中浸泡4天[C])诃子制草乌和生草乌配制嘎日迪-5混悬液的热板法镇痛作用、二甲苯致小鼠耳廓肿胀法抗炎作用。结果:对小鼠热板法致疼痛刺激反应比较结果为:在A条件炮制草乌配成的蒙药嘎日迪-5混悬液两个剂量组给药后30min、60 min时都有明显的镇痛作用(分别P<0.05和P<0.01)、B条件炮制草乌配成的蒙药嘎日迪-5混悬液高剂量组给药60 min以后不但没有镇痛作用反而有兴奋作用(P<0.05)、C条件炮制草乌配成的低、高剂量组给药60 min以后不但没有镇痛作用反而有兴奋作用(分别P<0.01、P<0.001)。对二甲苯致小鼠耳廓肿胀炎症的影响比较结果为:在A条件炮制草乌配成的蒙药嘎日迪-5混悬液两个剂量组都有明显的预防和治疗作用(P<0.05);B条件炮制草乌配成的蒙药嘎日迪-5混悬液高剂量组有明显的预防和治疗作用(P<0.01);C条件炮制草乌配成的蒙药嘎日迪-5混悬液没有明显的预防和治疗作用;生药组对小鼠热板法致疼痛刺激反应和对二甲苯致小鼠耳廓肿胀炎症都没有镇痛和抗炎作用。结论:初步研究证明在A条件炮制草乌配成的蒙药嘎日迪-5混悬液镇痛、抗炎作用最佳,此炮制方法和条件为诃子制草乌的进一步规范化研究和临床应用提供试验依据。

Objective： Dissimilar condition myrobalan system wild aconite enlightens - 5 to the anasthetic loud snapping sound date to ease the pain the anti - inflammation function influence. Methods ： Hot plate law experiment observation analgesia function; The xylene mouse auricle swelling inflammation model influence experiment observes the anti - inflammation function. Results： The anasthetic loud snapping sound date enlightens - 5 to soak in the myrobalan soup 12h, the decoction 2h concocts the condition next 30min, when 60min has the obvious analgesia function to the mouse hot plate legal system ache stimulation response （distinction P 〈 0.05 with P 〈 0.01 ）, the xylene sends the mouse auricle swelling inflammation to have the obvious prevention and the treatment function （ P 〈 0.05 ） ; Not only will soak 24h decoction 2h to concoct the condition the high monitoring team not to ease the pain in 60min later the function instead to have the excited function （ P 〈 0.05）, the xylene will send the mouse auricle swelling inflammation to have the obvious prevention and the treatment function （ P 〈 0.01 ） ; Soaks 4d not only low, the high monitoring team has not eased the pain in 60min later the function instead to have the excited function （ P 〈 0.01, P 〈 0. 001 ）, the xylene sends the mouse auricle swelling inflammation to have the obvious prevention and the treatment function ; The crude drugs group sends the mouse auricle swelling inflammation to the mouse hot plate legal system ache stimulation response and the xylene not to have the analgesia and the anti - inflammation function. Conclusion： The preliminary study proofs soaks in the myrobalan soup 12h, decoction 2h which under A condition the system wild aconite matches to enlighten - 5 analgesic anti - inflammation function is best, this concocts the method and the condition concocts the condition for the wild aconite the further research and the clinical practice provides the experimental basis.