蒙药达如奇颗粒“祛黏疫调理体素”治疗作用与调节巨噬细胞分泌炎性因子的实验研究

Experimental Study on Mongolian Medicine Daruqi Pellet "Removing Infected Visco Body-Conditioning" Treatment and Regulation of Macrophage Secretion of Inflammatory Factor

目的:探讨蒙药达如奇颗粒祛黏疫、调理体素的治疗机理与调节巨噬细胞分泌炎性因子IL-1、IL-6、TNF-α作用的相关性。方法:①50只BALB/C小鼠随机分为正常对照组,模型组,达如奇颗粒预防、低剂量、高剂量组,每组各10只。除正常对照组外,其余各组腹腔注射大肠杆菌菌液复制蒙医"黏疫"增盛热症模型。预防组于造模前48h开始灌胃每日1次;低、高剂量组分别于造模后2h灌胃达如奇颗粒混悬液0.60g/kg和1.20g/kg每日1次,连续3次。于末次灌胃2h后处死小鼠,抽取腹腔洗液,2000 r/m in,离心5m in。用ELISA法检测各组腹腔液中IL-1,IL-6及TNF-a的含量。②将上述腹腔液离心获得的腹腔单核-巨噬细胞培养36h,离心取上清液,检测IL-l、IL-6、TNF-α含量。结果:正常对照组与模型组比较,腹腔液和单核-巨噬细胞培养液中IL-1、IL-6、TNF-α的含量均有显著性差异(P<0.05);达如奇颗粒预防、低、高剂量组与模型组比较,腹腔液IL-1、TNF-α的含量明显降低(P<0.05);达如奇颗粒预防组、高剂量组与低剂量组比较,腹腔液IL-1的含量明显降低(P<0.05);达如奇颗粒预防、低、高剂量组与模型组比较,单核-巨噬细胞培养液中IL-6的含量明显降低(P<0.05)。结论:达如奇颗粒祛黏疫、调节体素的治疗作用与抑制炎性因子IL-1、IL-6、TNF-α的分泌有关,而且达如奇颗粒有很好的预防作用。

Objective： To discuss the ＂removing infected visco,body-conditioning＂ mechanism and regulation of macrophage secretion of IL-1,IL-6,TNF-α content of Mongolian medicine Daruqi Pellet.Method：①50 BALB/C mice into the normal control group,model group,Daruqi Pellet precaution group,low-dose group as well as high-dose group at random and each group has 10 mice.In addition to the normal control group,other group are given colibacillus fluid,to make mould of traditional Mongolian medicine ＂microbes＂ hyperpyrexia model.Before 48 hours being mould,Daruqi Pellet precaution group should be given intragastric administration once a day.After 2 hours being mould,low-dose group,high-dose group should be given respectively intragastric administration suspension of Daruqi Pellet 0.60g/kg,1.20g/kg once a day,continue to have 3 times.Two hours after the last time,the mice should be killed,to get the peritoneal fluid at 2000r/min,centrifugal 5 minutes,using ELISA method to test the quality of IL-1,IL-6 and TNF-α.②At the centrifugate to get the monocytes-macrophages,cultivanting 36 hours to test the quality of IL-1,IL-6 and TNF-α.Result：Compared the normal control group with model group,the quality of IL-1,IL-6 and TNF-α in Peritoneal fluid and monocytes-macrophage medium has great difference（P﹤0.05）;Compared Daruqi Pellet precaution group,low-dose group as well as high-dose group with model group,the quality of IL-1 and TNF-α in peritoneal fluid is apparent low（P﹤0.05）;Compared Daruqi Pellet high-dose group with low-dose group,the quality of IL-1 in Peritoneal fluid deduced（P﹤0.05）.Compared Daruqi Pellet precaution group,low-dose group and high-dose group with model group,the quality of IL-6 in monocytes-macrophage medium is apparently low（P﹤0.05）;Conclusion：The treat fuction of removing infected visco,adjusting voxel of Daruqi Pellet may be relationg to Inhibiting secretion of inflammatory factors（IL-1,IL-6,TNF-α） and Daruqi Pellet is good to prevent effect.