间歇低氧对幼鼠纹状体边缘区p38MAPK和超微结构的影响及芹菜素的干预作用

The Infusion of Intermittent Hypoxia on p38MAPK and UItrastructure Changes in Marginal Striatum of Weanling Rats and the Intervention Effects of Apigenin

目的:研究间歇低氧对幼鼠纹状体边缘区p38MAPK及超微结构的影响及芹菜素的干预作用。方法:SPF级健康雄性SD幼鼠(3~4周龄)40只,随机分为4组:间歇低氧2周组(IH组)、对照组(C组)、芹菜素+二甲基亚砜组(Q组)和二甲基亚砜组(R组)。建立间歇低氧幼鼠模型,以RT-PCR法和Westernblot法分别测幼鼠纹状体内侧边缘区p38MAPK mRNA和磷酸化p38MAPK(p-p38)蛋白的表达,并电镜观察超微结构的改变。结果:IH组幼鼠纹状体边缘区的p38MAPK mRNA及p-p38蛋白均明显高于C组(P均<0.01),Q组的表达则明显低于R组(P均<0.01),电镜下IH组和R组纹状体边缘区组织细胞核膜模糊不清晰,染色质浓缩、边聚,线粒体肿胀空泡化,Q组和C组无明显变化。结论:间歇低氧可激活幼鼠脑区p38MAPK,进而引起超微结构的改变,而芹菜素可至少部分抑制这种激活,这可能对减轻间歇低氧后纹状体边缘区等脑区损伤具有积极意义。

Objective：To explore the infusion of intermittent hypoxia on p38MAPK and ultrastructure changes in marginal striatum of weanling rats and the intervention effects of apigenin.Methods：Randomly,fourty male Sprague-Dawley rats（3-week.old^4-week-old）were divided into four groups：2-weeek-IH group（IH）,control group（C）,apigenin＋DMSO（Q）group and DMSO control（R）group.Intermittent hypoxia model was induced by an intermittent hypoxia cabin.In medial border of the striatum,the marginal division（MrD）,the expression of p38MAPK mRNA and the phosphorylation levels of p38MAPK（phospho-p38）were measured by RT-PCR or Westernblot respectively.The ultrastructure of MrD was studied by electron microscope.Results：In the MrD,the expression of p38MAPK mRNA and p-p3 8 protein in IH group were respectively higher than C group（P〈0.01,respectively）.These expressions in Q group had significantly less than in R group（P〈0.01,respectively）.The nuclear membrane of MrD cells in IH or R groups were broken,chromatin concintration and edge accumulation and some mitochondria were enlarged and vacuoled.Conclusions：Intermittent hypoxia actived the p38MAPK signal transduction,led ultrastructure of neuron disordered in brain regions.Apigenin can partial reduce the activation of p38MAPK which may play a positive role in alleviating the cerebral tissues injury in intermittent hypoxia weanling rats.