督脉配穴对染铅大鼠脑中NO和NOS保护作用研究

Protective Effects of Dumai Peixue on NO and NOS in Lead-exposed Rats

目的:研究督脉配穴对染铅大鼠脑中NO和NOS保护作用。方法:40只SD大鼠随机分为4组,即正常组、染铅模型组、观察组(督脉配穴组)和对照组。除空白组外,其余3组腹腔注射醋酸铅染铅。观察组和对照组给予针灸治疗,其它组自由进食与饮水,实验周期为4周。实验开始后第2周和第4周就行水迷宫实验,实验结束后测血铅浓度及海马中NO、NOS含量。结果:针灸治疗对血铅浓度无显著影响,P>0.05;治疗后大鼠达到水迷宫平台区的时间明显缩短,P<0.05;治疗后因染铅而降低的海马中N0、NOS含量升高,差异具有显著性,P<0.05。结论:针灸治疗对染铅大鼠海马有一定保护作用。

Objective：To study the protective effects of Dumai peixue on NO and NOS in lead-exposed rats.Methods：40 male SD rats were randomly divided into four groups：normal group,model group,observered Group（Dumai peixue）and the control group.Except the normal group,the other three groups were exposed to lead through injecting lead acetate.The observered group and the control group were given acupuncture treatment,other groups eating and drinking freely.The experimental period was 4 weeks.Two and four weeks after the experiment,Morris water experiment was used to detect the time arriving at the platform.At the end of the experiment.measured the content of blood lead and concentrations of NO and NOS in hippocampus.Results：Acupuncture treatment had no significant effects on blood lead concentrations.P〉0.05.The time achieving at the platform was shorten significantly after treatment.P〈0.05;the concentration of NO and NOS in hippocampus decreased significantly after the treatment.P〈0.05.Conclusion：Acupuncture treatment has a protective effect on hippocampus in lead-exposed rats.