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实时定量PCR芯片——功能基因组研究的有用工具 被引量：3

Quantitative PCR array:a useful tool for functional genomic studies
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摘要 结合实时定量PCR(real-time quantitative polymerase chain reaction,qPCR)和基因芯片技术,实时定量PCR芯片(qPCR array)为功能基因组研究提供了一种高度灵敏、可靠的方法。在基因功能分类基础上,结合qPCR准确定量的特性,qPCR array可同时定量分析上百个基因的mRNA表达水平,是研究一组特定功能基因表达的理想方法。 Combined with two techniques of real-time quantitative polymerase chain reaction （qPCR） and gene chips, the qPCR array brings a highly sensitive and reliable method of gene expression profiling. It allows quantitatively monitoring expression levels of panel genes in a specific pathway or disease related genes simultaneously, and is a useful tool for functional geneomic studies recently.
作者 曲佳 熊鸣 赵晓航
机构地区 军事医学科学院 海军总医院 中国医学科学院肿瘤研究所
出处 《生命科学》 CSCD 北大核心 2009年第2期241-245,共5页 Chinese Bulletin of Life Sciences
基金 总后医药卫生科研计划(06MB022) 海后司令部科研计划(07-3309) "973"项目(2004CB518707)
关键词 实时定量PCR芯片 信号通路分析 基因表达 qPCR array signal transduction analysis gene expression
分类号 Q503 [生物学—生物化学] Q78 [生物学—分子生物学]
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参考文献13

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二级参考文献14

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共引文献7

同被引文献57

  • 1章金涛,王纯耀,康巧珍,凌雁,金树兴,祝庆蕃.昆明种无毛小鼠及C57BL/6无毛小鼠皮肤组织学比较研究[J].中国实验动物学杂志,1997,7(1):4-6. 被引量：10
  • 2遇秀玲,李善如,吴娜.被毛突变无毛小鼠皮肤及毛结构观察[J].中国兽医学报,1996,16(3):299-301. 被引量：8
  • 3戴亚斌,何农跃,刘梅.基因芯片技术及其在微生物检测中的应用[J].动物医学进展,2006,27(10):41-46. 被引量：8
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二级引证文献9

生命科学
2009年 第2期

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