摘要
目的观察α-鹅膏毒肽(α-Amanitin)对HepG22.2.15细胞(2.2.15细胞)乙肝病毒(HBV)HBsAg和HBeAg分泌的影响。方法用不同浓度的α-鹅膏毒肽作用2.2.15细胞,用MTT法检测细胞毒性,时间分辨免疫荧光法(TRFIA)测定上清HBsAg和HBeAg浓度。结果α-鹅膏毒肽在0.006~0.800μg/mL时,对HepG22.2.15细胞无明显毒性作用,当浓度达到4.000~20.000μg/mL时毒性较明显,TC50为10.190μg/mL;其对上清液HBsAg和HBeAg的抑制随浓度的增加而加强,其半数抑制浓度分别为0.495μg/mL和0.346μg/mL。结论在无毒或低毒浓度下α-Amanitin能抑制2.2.15细胞株HBsAg和HBeAg的分泌,可能与α-Amanitin抑制HBVDNA的复制有关。
[Objective] To estimate the cytotoxicity of α-amanitin to 2.2.15 cell lines and then to investigate the inhibitory influence of α-Amanitin on the secretion of HBsAg and HBeAg to 2.2.15 cell lines. [Methods] HepG2 2.2.15 ceils were incubated for 3 days with DMEM culture medium containing different concentration of α-Amanitin and 10% fetal bovine serum,the growth inhibitory effect of α-Amanitin on 2.2.15 cell lines was examined by the MTT assay, the concentration of HBsAg and HBeAg in the culture medium were accessed by TRFIA. [Results] No or little significant cytotoxicity of ot-Amanitin to 2.2.15 cell lines was observed in the concentration ranging from 0.0064 μg/mL up to 0.8 μg/mL, while significant cytotoxieity was founded in the concentration ranging from 4 μg/ mL up to 20 μg/mL with a TC50 value of 10.19 μg/mL. The concentrations of HBsAg anti HBeAg of 2.2.15 cell lines treated by α-amanitin were decreased compared with negative control group ,with an IC50 value of 0.495 μg/ mL and 0.346 μg/mL, respectively. The inhibitory effection was enhaced with the increased concentration of it. [Conclusions] At the no or little cytotoxicity concentration, α-Amanitin could suppress the secretions of HBsAg and HBeAg of 2.2.15 cell lines, which suggested that it maybe eorrelated with inhibiting the duplication of HBV- DNA by α-Amanitin.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2009年第7期973-976,共4页
China Journal of Modern Medicine
基金
湖南省计划发展委员会资助项目(2002年)