EZH2基因在乳腺癌组织中的表达及其意义

Expression and clinical significance of EZH2 in breast cancer tissue

目的探讨EZH2在乳腺癌组织中的表达及其与临床病理的关系。方法半定量PCR法检测EZH2mRNA表达,免疫组化SP法检测EZH2的蛋白表达情况,分析其表达与临床病例特征的关系。结果肿瘤组织中EZH2mRNA和EZH2蛋白的表达水平均高于正常乳腺组织(P<0.05);EZH2在肿瘤组织的mRNA和蛋白的表达与肿瘤的病理学分级、淋巴结转移有关(P<0.05),与年龄、临床分期等病理参数无关。结论乳腺癌组织EZH2基因表达增加与乳腺癌恶性生长及不良预后有关。

[Objective] To investigate the expression of EZH2 in breast cancer tissue and relationship with clinieopathological parameters. [Methods] The expression level of EZH2 mRNA and protein in breast cancer tissue was detected with reverse transeription-polymerase chain reaction （RT-PCR） and immunohistoehemical method respec- tively. We analyzed the relationship between EZH2 expression and elinicopathological parameters. [Results] There is significandy difference in expression of EZH2 mRNA and protein between malignant tissues and non cancerous tissues （P 〈0.05）. The expression of EZH2 mRNA and protein in malignant tissues correlated with pathological grade and node metastasis and was not related to other parameters including age and clinical stage. [Conclusion] Increased expression of EZH2 in breast cancer tissue play an important role in malignant growth and poor prognosis of breast cancer.