高脂、胰岛素抵抗合并高脂高糖对大鼠肝脏Angptl3和LPL基因mR-NA转录的影响

Effect of Hyperlipidemia and Hyperlipidemia with High Glucose Insulin-Resistant on Transcription of Angptl3 and LPL mRNAs in Rat Liver

目的探讨高脂、胰岛素抵抗合并高脂高糖对大鼠肝脏血管生成素样蛋白3(Angptl3)和脂蛋白脂肪酶(LPL)基因mRNA转录的影响。方法建立高脂(HL)、胰岛素抵抗合并高脂高糖(IR-HLG)大鼠模型,采用自动分析仪检测模型大鼠空腹血糖(BG)、总三酰甘油(TG)和胆固醇(TC)含量,采用Realtime PCR定量检测各组大鼠肝组织Angptl3和LPL基因的mRNA转录水平。结果模型组大鼠血清TG、TC含量及肝脏Angptl3和LPL基因mRNA转录水平均较对照组明显升高;IR-HLG组大鼠的BG水平和Angptl3水平较对照组和HL组均明显升高,LPL水平较HL组明显下降。结论随血脂的增高,Angptl3和LPL的表达均增强;在高糖、胰岛素抵抗状态下,Angptl3的表达增高,而LPL的表达则部分受到抑制。

Objective To explore the effect of hyperlipidemia（HL）and hyperlipidemia with high glucose insulin-resistant （IR-HLG）on the transcription of angiopoietin-related protein 3（Angptl3）and lipoprotein lipase（LPL）mRNAs in rat liver. Methods The rat models of HL and IR-HLG were established and determined for blood glucose（BG）,triglyeride（TG）and total cholestero（lTC）by automatic analyzer,and the transcription levels of Angptl3 and LPL mRNAs in liver tissue by real-time quantitative PCR. Results The TG and TC contents in sera as well as transcription levels of Angptl3 and LPL mRNAs in liver tissue of model rats were significantly higher than those of normal control. The BG and Angptl3 levels of rats with IR-HLG were significantly higher than those of normal control and those of rats with HL,while LPL level was significantly lower than that of rats with HL. Conclusion The expressions of Angptl3 and LPL increased with increasing blood fat content. Under the condition of high blood glucose and insulin-resistance,the expression of Angptl3 increased,while that of LPL was partly inhibited.