鄱阳湖具刚毛荸荠居群遗传多样性和克隆结构的初步研究

Preliminary Studies on Genetic Diversity and Clonal Structure of Heleocharis valleculosa f. setosa Populations in Poyang Lake

采用ISSR标记对鄱阳湖的2个具刚毛荸荠(Heleocharis valleculosa f.setosa)居群共120个个体的遗传多样性和克隆结构进行了研究。5条ISSR引物共检测到85个位点,其中49个为多态位点,占57.65%。具刚毛荸荠具有较高的遗传多样性(在物种水平上,Nei’s基因多样性指数H=0.1545,Shannon信息指数I=0.2400),两居群间遗传分化很小。具刚毛荸荠的克隆多样性很高(D=0.9975),两居群不具有共同的基因型。空间自相关分析表明,具刚毛荸荠居群内遗传变异为随机分布格局。

By using inter-simple sequence （ISSR） markers, this paper studied the genetic diversity and elonal structure of two Heleoeharis valleeulosa f. setosa populations （120 individuals） in Poyang Lake. By five primers,85 fragments were amplified ,of which 49 （57.65%） were polymorphic. As a result, a relatively high level of genetic diversity was revealed （ H = 0. 1545, I = 0. 2400, at species level）, but a low level of genetic differentiation was detected between the two populations （ Gsr = 0. 0201 ）. High clonal diversity was detected but no common genotype found between the two populations, which meant great clonal differentiation. In both populations, the spatial genetic structure were found as a random mode.