顺铂联合人参皂甙Rg3对荷宫颈癌鼠血管生成的抑制作用

Anti-angiogenic Effects of Cisplatin Combined with Ginsenoside Rg3 in Nude Mice Cervical Cancer

目的探讨人参皂甙Rg3(简称Rg3)与顺铂联合应用对人宫颈癌动物模型中肿瘤的生长及其对肿瘤血管生成的影响。方法采用人宫颈癌HeLa细胞株接种于28只雌性裸鼠,随机均分成4组:即(1)生理盐水对照组;(2)顺铂(DDP)组;(3)人参皂甙Rg3组;(4)人参皂甙Rg3与顺铂联合组。各组用药时间均为5周,35天后脱颈处死。分别检测肿瘤的重量,并计数肿瘤内微血管密度(MVD)。结果Rg3组、DDP组及联合组对原位种植肿瘤的生长有明显的抑制作用,联合治疗组抑制肿瘤生长的作用明显优于单纯组;且Rg3及联合治疗组MVD也明显低于DDP组及对照组。结论Rg3与DDP联合应用能抑制人移植性宫颈癌的生长,降低肿瘤内的MVD,抑制宫颈癌肿瘤血管生成。

Objective To study the anti-angiogenic effects and anti-tumor effect of cisplatin combined with ginsenoside Rg3 in nude mice cervical cancer. Methods 28 female nude mice were randomly divided into 4 groups to receive saline （0. 5 ml, qd）, Rg3（5 mg/kg） alone, cisplatin （5 mg/kg） alone and Rg3 （5 mg/kg） combined with cisplatin （5 mg/kg） respectively. Rg3 was given by gastrogavage once a day, for 5 weeks, cisplatin 3 was injected introperitoneally once 4 days for 5 weeks. After treatments for 5 weeks,mice were killed and the sizes of solid tumors were measured, the intratumor MVD （microvessel density） was examined by immunohistochemical staining. Results The tumor weights of treated groups were significantly lower than those of the control group, while the tumor weights of Rg3 combined with cisplatin group were the lowest. The MVD value of Rg3 group were significantly lower than that of cisplatin group and control group. Conclusion Rg3 combined with cisplatin can significantly inhibit the growth of transplanted human cervical cancer in nude mice. Rg3 can obviously reduce the intratumoral MVD by inhibiting angiogenesis of malignant tumor.