期刊文献+

甘糖酯对体外培养的晶体上皮细胞增殖的影响 被引量:4

EFFECT OF PGMS ON THE PROLIFERATION OF LENS EPITHELIAL CELLS IN CULTURE
下载PDF
导出
摘要 后发性白内障主要是由于术后残留的晶体上皮细胞移行到后囊并增殖所致。用培养的新生大鼠晶体上皮细胞实验,以结晶紫染色和~3H-TdR掺入作指标,表明甘糖酯(0.2~0.8mg/ml)对细胞增殖有明显抑制作用,且比同浓度肝素作用略强。提示甘糖酯对防治后发性白内障可能有效。 Delayed opacification of the posterior capsule is the most common cause of decreased visual acuity after extracapsular cataract extraction. It has been shown to result from migration and proliferation of residual lens epithelial capsule. The effect of proglylene glucol mannate sulfate (PGMS, a polysaccharide sulfate) on the proliferation of cultured newborn rat LECs was investigated with cell culture method. LECs were incubated in modified Medium 199 at 37C in 5%CO2,and the first or second passage cells were used. The cells were exposed to 0. 2,0. 4,0. 8 mg/ml concentrations of PGMS or heparin,compared with normal control,for 72h to measure their proliferation rates by crystal violet staining and colori-metric analysis ,and 24h to account for an inhibion of 3H-TdR incorporation. The results imply that PGMS can obviously inhibit the proliferation of rat LECs in vitro(P<0. 05). PGMS, capable of inhibiting lens epithelial cells growth,if free of unacceptable toxic effects on other cell populations,might be used to prevent lens capsule opacification.
出处 《中国海洋药物》 CAS CSCD 1998年第1期24-28,共5页 Chinese Journal of Marine Drugs
关键词 甘糖酯 肝素 晶体上皮细胞 后发性 白内障 proglylene glucol mannate sulfate (PGMS) heparin lens epithelial cells (LECs) after-cataract
  • 相关文献

参考文献12

  • 1Goodman D F,Stark W J,Gottsch J D. Complications of cataract extraction with intraocular lens implantation. Ophthalmic Surg, 1989; 20(2) : 132.
  • 2Zaturinsky B,Naveh N,Saks D,et al, Prevention of posterior capsular opacification by cryolysis and the use of heparinized irrigating solution during extracapsular lens extraction in rabbits.Ophthalmic Surg,1990;21(6):431.
  • 3夏小平,陆道炎,王丽天,陈才根,吴念祖.肝素抑制后发性白内障形成的实验研究[J].眼科新进展,1994,14(4):6-7. 被引量:3
  • 4Rind H,Vornhagen R,Koch HR. Rat lens epithelial cells in vitro. In Vitro,1980;16(1):15.
  • 5Castellot JJ, Cochran DL, Karnovsky M, Effect of heparin on vascular smooth muscle cells. J Cell Physiol,1985;124:21.
  • 6McDonnell PJ,Krause W,Glaser BM. In vitro inhibition of lens epithelial cell proliteration and migration. Ophthalmic Surg 1988;19 (1) : 25.
  • 7Power WJ,Neylan D,Collum LMT. Daunomycin as an inhibitor of human lens epithelial cell proliferation in culture. J Cataract Refract Surg,1994;20:287.
  • 8Legler UFC,Apple DJ,Assia EI,et al. Inhibition of posterior capsule pacification: The effect of colchicine in asustained drug delivery system. J Cataract Refract Srug, 1993;19:462.
  • 9夏小平,陆道炎,王丽天,陈才根,吴念祖.肝素抑制后发性白内障形成的临床研究[J].中华眼科杂志,1994,30(6):405-407. 被引量:27
  • 10姜国辉,张世玲,袁玮,朱嫒嫒,杨丛秀.甘糖酯对家兔凝血和纤溶功能的影响[J].中国药理学通报,1991,7(3):201-204. 被引量:12

共引文献38

同被引文献39

引证文献4

二级引证文献30

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部