nm23-H1杂合性等位基因丢失与肝癌转移的关系

The relationship between nm23 H1 loss of heterozygoisty and metastasis in hepatocellular carcinoma

目的从ＤＮＡ水平揭示ｎｍ２３－Ｈ１杂合性基因丢失与人原发性肝癌肝内转移、门静脉形成癌栓等的临床病理特征的关系。方法应用Ｓｏｕｔｈｅｒｎ印迹杂交技术，对２５例肝癌及癌旁肝组织ＤＮＡ进行分析。结果ｎｍ２３－Ｈ１两条等位基因分别为７．６Ｋｂ、２．３Ｋｂ，杂合性等位基因丢失率为３１．２５％（５／１６）。ｎｍ２３－Ｈ１杂合性基因丢失多见于分化较差的ＥｄｍｏｎｄｓｏｎⅢ、Ⅳ级和伴肝内转移或门静脉形成癌栓的肝癌。结论ｎｍ２３－Ｈ１可能诱发肝细胞肝癌的转移潜能，有助于预测肝癌复发和转移。

Objective To demonstrate the relationship between nm23 H1 loss of heterozygoisty (LOH) and clinical pathological characteristics of hepatocellular carcinoma(HCC) from the DNA level. Method Southern blot hybridization was used to analyze genomic DNA in liver cancer and its corresponding liver tissue. Result nm23 H1 has diallelic bands at 7 6 Kb and 2 3 Kb.Allelic loss of heterozygoisty in tumour tissues was detected in 31 25% patients (5 patients).LOHs were more common in the tumours with intrahepatic metastasis or portal vein tumour emblus and the tumours poorly differentiated(Edmondson's classificasionⅢ、Ⅳ). Conclusion nm23 H1 LOHs may induce the metastasis potential of HCC and help to predict recurrence and metastasis of HCC.