应用聚合酶链反应探讨乙肝病毒感染与胆石症的关系

PCR in detecting the correlation between infection of HBV and cholelithiasis

目的探讨乙肝病毒感染在胆石症发病中的作用。方法应用聚合酶链式反应技术（ＰＣＲ），对３２例血清乙肝标志物阳性的胆石症患者和２０例乙肝标志物阳性的非胆石症患者的石蜡包埋胆囊标本进行ＨＢＶ－ＤＮＡ检测。结果３２例胆石症患者的胆囊标本ＨＢＶ－ＤＮＡ阳性检出率为４０．６３％，２０例非胆石症患者的胆囊标本ＨＢＶ－ＤＮＡ阳性检出率为１５％。结论提示乙肝病毒感染与胆石症发生有关。用ＰＣＲ技术检测石蜡包埋胆囊组织中的ＨＢＶ－ＤＮＡ是一种简便、可靠的方法。

Objective To study the correlation between cholelithiasis and the infection of HBV. Method 32 formalin fixed and paraffin embedded gallbladder samples of cholelithiasis patients and 20 gallbladder samples of noncholelithiasis patients were investigated using the polyme rase chain reaction Ethidium bromide(PCR EB)assay.The 52 patients were positive to HBV serologic markers. Result The results showed that HBV DNA was found in 13 gallbladder samples of 32 cholelithiasis patients(40 63%),significantly higher than that in 3 gallbladder samples of 20 noncholelithiasis patients(15%). Conclusion The infection of HBV and the formation of cholelithiasis are correlated.