肝细胞、胰岛细胞肝内移植对肝硬变影响的研究

The influence of intrahepatic transplantation of hepatocytes and insular cells on liver cirrhosis

目的避免脾切除、门腔分流术后所致向肝血流减少造成的肝功能损害。方法白蛋白将分离纯化的肝细胞、胰岛细胞经肝动脉灌注肝内移植。结果细胞组与对照组比较血总蛋白、白蛋白、胆红素改善程度，差异有极显著意义（Ｐ＜０．０１）。肝纤维化光镜检查及Ⅰ、Ⅲ型胶原免疫组织化学染色及图象分析结果，差异有显著意义（Ｐ＜０．０５）。结论肝细胞、胰岛细胞经肝动脉灌注肝内移植，不但能改善肝功能，还能促进肝脏胶原纤维降解，逆转肝纤维化、肝硬变的程度。

Objective To protect liver function, we used the cirrhotic rats to study whether the postshunt intrahepatic transplantation of hepatocytes and insular cells via hepatic artery can improve liver funcyion. Method We irrigated the cirrhotic liver of rat via hepatic artery with hepatocytes and insular cells and observed the influence of this method on liver function. Result The improvement of TP, ALB and TBIL in the experimental group were significant, and there was significant difference in these paremeters between the experimental and control groups( P <0 01). Light microscopic examination of liver fiberosis and immunohisochemistry and data analysis of Ⅰ, Ⅱ collagens also showed significant difference( P <0 05). Conclusion The inttrahepatic transplantation of hepatocytes and insular cells via hepatic artey can not only improve liver fuuction, but also incerase the degradation of liver collagens and reverse the coure of liver cirrhosis.