血管紧张素原基因 M235T 分子变异与冠心病的关系

A MOLECULAR VARIANT OF ANGIOTENSINOGEN GENE IS ASSOCIATED WITH CORONARY HEART DISEASE IN CHINESE

目的研究血管紧张素原（ＡＧＴ）基因Ｍ２３５Ｔ分子变异与中国人冠心病（ＣＨＤ）发生的关系。方法采用多聚酶链反应（ＰＣＲ）、限制性片段长度多态性分析，对７６例ＣＨＤ患者和７６例无ＣＨＤ证据的对照组进行ＡＧＴ基因Ｍ２３５Ｔ等位基因检测。结果ＣＨＤ患者ＡＧＴ基因２３５ＴＴ型（０．７０）和Ｔ２３５等位基因（０．８２）的频率显著高于对照组（分别为０．４２和０．６３，Ｐ＝０．００２和Ｐ＜０．０１）。经校正ＣＨＤ的主要危险因素后，ＡＧＴ基因２３５ＴＴ型仍可显著增加ＣＨＤ发生的危险性（比数比为３．５６，Ｐ＝０．０１２）。结论中国人ＡＧＴ基因Ｍ２３５Ｔ分子变异与ＣＨＤ显著相关，ＡＧＴ基因２３５ＴＴ型可能是ＣＨＤ的重要危险因素之一。

URPOSE To study whether a molecular variant of the angiotensinogen (AGT) gene with threonine instead of methionine at position 235 (i.e., with M235T polymorphism) was related with coronary heart disease (CHD) in a Chinese population.METHODS M235T polymorphism in exon 2 of AGT gene was determined by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) in 76 patients with CHD (75% of MI) and 76 non-CHD subjects as control.RESULTS The frequencies of T235 allele (0.82) and 235TT genotype (0.70) were higher in the CHD group than that of the control group (0.63 and 0.42 P=0.002 and P<0.01 respectively). AGT gene 235TT genotype was shown to significantly increase. The risk of CHD (odds ratio 3.56, P=0.012) in analysis adjusted for several main CHD risk factor.CONCLUSIONS There is a significant relationship between AGT gene 235TT genotype and CHD. This genotype might be an independent risk factor for CHD in Chinese population.