肿瘤细胞 DNA 倍体和 P53 蛋白表达分析

P53 PROTEIN EXPRESSON AND DNA CONTENT IN THE CANCER CELL

目的探索Ｐ５３蛋白表达与ＤＮＡ倍体以及与其他临床指标的关系。方法我们用流式细胞仪双参数检测了７１例临床肿瘤手术标本的ＤＮＡ含量和Ｐ５３蛋白表达。其中良性肿瘤８例，卵巢癌４２例，肺癌１２例，乳腺癌９例。同时用免疫组化方法检测上述样本３２例的石蜡切片，作Ｐ５３蛋白表达检测。结果良性肿瘤８例，６例（７５％）两倍体，２例（２５％）异倍体，Ｐ５３均为阴性（１００％）。恶性肿瘤６３例，２２例两倍体，其中４例（１８％）Ｐ５３阳性，１８例（８２％）Ｐ５３阴性。４１例异倍体，其中２１例（５１％）Ｐ５３阳性，２０例（４９％）Ｐ５３阴性。经统计分析，免疫组化ＡＢＣ法和流式细胞仪法Ｐ５３蛋白表达检测两者有明显一致性（Ｐ＜０．００１）。Ｐ５３高表达与ＤＮＡｉｎｄｅｘ明显正相关（Ｐ＜０．００５）。在肿瘤良恶性，临床分期、病理分级比较中，Ｐ５３阳性表达只与ＤＮＡ异倍体密切相关（Ｐ＜０．０２５），与其他临床指标不相关。结论Ｐ５３阳性表达与ＤＮＡ异倍体密切相关，可能是肿瘤发生的机制之一。

URPOSE To explore the relationship between P53 protein expression, DNA ploidy and clinical characters in the cancer cells.METHODS P53 protein expression and DNA content were examined in 71 cases obtained from 71 patients surgically treated (8 Benign tumor; 42 Ovarian cancer; 12 Lung cancer; 9 Breast cancer) for bivariate flow cytometric analysis. P53 protein expression was detected immunohistochemically in the same 32 cases in paraffin-embedded tissue.RESULTS We investigated that results of these two methods were correlated with P53 protein expression (P<0.001). Overexpression of P53 protein was observed in 21 (51%) aneuploid cancer and 4 (18%) diploid cancer (P=0.025). None of the eight samples of benign tumor was positive compared with malignant cells. The frequency of P53-positive was associated with DNA index (P<0.005).CONCLUSIONS The results show that DNA ploid pattern and P53 expression are closely related. DNA content and P53 expression are significant in clinical treatment by FCM.