191例慢性乙型肝炎患者乙型肝炎病毒YMDD突变型核酸检测结果及分析

The analyse and the result that chronic heoatitis B patient take place the variation of YMDD detectioned with PCR

目的 慢性乙型肝炎患者服用拉米夫定后其乙型肝炎病毒YMDD的变异结果分析。方法采用FQ-PCR技术检测临床血清或血浆样本中的乙型肝炎病毒DNA，并同时确定其基因为野生型（YMDD）或耐药突变型（YVDD／YIDD）。结果191例慢性肝炎患者中，有YMDD变异的81例，其中有69例患者服用过拉米夫定。结论服用拉米夫定的患者其YMDD变异明显高于未服用拉米夫定的患者，二者差异有统计学意义（P〈0．01）。

Objective The analysis is that chronic hepatitis B patient take place the variation of YMDD after taking the 1 amivudine. Methods Using FQ-PCR technique examine hepatitis B virus DNA in the serum, make sure this gene belongs to the wild type （YMDD）or mutation types （YIDD YVDD）. Results There are 81 out of 191 eases having occurrence YMDD variation, of these,69 cases have been in patients with lamivudine. Conclusion The YMDD variation in sufferer who take the Lamivudine is obvious higher than did not take the Lamivudine sufferer（P 〈 0. 01 ）.