睾酮对人单核细胞中雄激素受体基因表达的影响

Effect of Testosterone on Gene Expression of Androgen Receptor in THP-1

目的:研究不同浓度的睾酮对人单核细胞中雄激素受体(AR)基因表达的影响。方法:对培养的人单核细胞株THP-1予不同浓度的睾酮刺激,采用RT-PCR法检测细胞中AR基因表达量,进行半定量比较。结果:人的单核细胞中存在AR。刺激3h后,睾酮浓度为10-6mol/L、10-5mol/L组和刺激6h后10-9～10-5mol/L的各组AR基因表达较对照组表达量明显减少,差异有统计学意义(P<0.05),给氟他胺(10-5mol/L)组和同时给予10-7mol/L睾酮组与对照组比较,AR的表达量差异无统计学意义(P>0.05)。结论:在一定范围内,睾酮可减少人单核细胞中AR的表达,选择性受体阻滞剂氟他胺可使这种作用完全阻滞。

Objective： To study the effect of testosterone on gene＇s expression of androgen receptor （AR） in THP-1. Methods： Cultured THP-1 was stimulated by different concentrations testosterone. The gene expression of AR was examined with RT-PCR. Results： ARs existed in human monocyte. Compared with the quantity of AR＇s expression in control group, those in 3 hours＇ stimulation by 10^-6 mol/L and 10^-5 mol/L groups and 6 hours＇ stimulation by 10^-9 mol/L to 10^-5 mol/L testosterone groups were obviously reduced. There was a significant difference between them（P 〈 0.05 ）. There was no significant difference between control group and being administered both flutamide and 10^-7mol/L testosterone group（P 〉 0.05）. Conclusion： The gene expression of androgen receptor can be reduced by testosterone at some range, and this reduction was completely abolished by a selective androgen receptor blocker flutamide