跟腱切断法诱导大鼠异位骨化动物模型的建立

Animal model of heterotopic ossification induced by Achilles' tenotomy in Sprague-Dawley rat

[目的]探讨建立一种异位骨化(heterotopie ossification,HO)动物模型,并初步研究其形成机制。[方法]36只雄性Sprague-Dawley(SD)大鼠随机分为三组(n=12):单纯切开皮肤组(sham)、跟腱半切组(partial A-chilles’tenotomy,PAT)、跟腱全切断组(Achilles’tenotomy,AT),于术后6、10周分别行跟腱X线和组织学检查,观察异位骨形成情况。[结果](1)sham和PAT组6、10周均未发生HO;(2)AT组:术后6周,3只大鼠X线片可见异位骨,3只组织学检查发现有软骨形成;术后10周,6只大鼠均出现HO。这种异位骨具有骨小梁和骨髓结构,是通过软骨化骨形成。[结论]跟腱切断术能有效诱导大鼠HO,该方法简单、有效、易行,结果稳定。

[Objective]To establish an animal model of heterotopie ossification （HO） induced by Achilles＇ tenotomy in Sprague-Dawley （SD） rat and to investigate its possible mechanism. [ Method] Thirty six male SD rats were divided into 3 groups （ n = 12 ）. In sham group, incision and suturation were performed only in the left leg. Partial tenotomy was performed in the left Achilies tendons in PAT group. In AT group,tenotomy was performed in the left Achilles tendons to establish animal model of HO. X-ray and histological examinations were made at 6 and 10 weeks after operation. [ Result] （ 1 ） No HO occurred in the sham and PAT groups. （2）In AT group,3 rats showed cartilage and bone formation on X-rays while the remaining 3 rats showed chondrifi- cation in histological examination at 6 weeks after operation. At 10 weeks all rats showed bone formation with trabecular bone. This kind of HO developed through a process of endochondral ossification. [ Conclusion ] Tenotomy is a simple, effective and feasible method to induce HO.