血管紧张素-（1-7）对转化生长因子-β1诱导系膜细胞增殖及细胞外基质分泌的影响

Effect of angiotensin- （ 1-7 ） on proliferation and secretion of cultured rat＇s glomerular mesangiai cells induced by transforming growth factor-β1

目的探讨血管紧张素-（1-7）[Ang-（1-7）]对转化生长因子-β1（TGF-β1）诱导大鼠系膜细胞（GMC）增殖和细胞外基质分泌的影响。方法选择对数生长期GMC，分为对照组、Ang-（1-7）组、TGF-β1组、TGF-β1＋Ang-（1-7）组，采用WST-1检测系膜细胞增殖，RT-PCR法和放射免疫法检测细胞外基质层粘蛋白（LN）、Ⅳ型胶原（COLIV）的表达。结果与对照组相比，Ang-（1-7）组细胞数目和细胞外基质减少（P〈0．05）；与TGF-β1组相比，Ang-（1-7）可抑制TGF-β1诱导的系膜细胞增殖、使细胞内LNmR-NA、COLⅣ mRNA表达量明显下调、同时导致上清液中细胞外基质LN、COLⅣ含量显著减少（P〈0．05）。结论Ang-（1-7）能抑制基础和TGF-β1诱导的GMC细胞增殖及细胞外基质的分泌，可能在抑制肾脏纤维化过程中发挥作用。

Objective To explore the effect of angiotensin-（ 1-7 ） [ Ang-（ 1-7 ） ] on proliferation and secretion in cultured rat＇s glomerular mesangial cells（GMC） induced by transforming growth factor-β1 （TGF-β1）. Methods GMC in logarithmic growth phase were incubated, and then were divided into 4 groups： control, Ang-（ 1-7）, TGF-β1,and TGF-β1 ＋ Ang-（1-7） group. Cell numbers of rat＇s GMC were detected by WST-1. Laminin（LN） and collagenⅣ （ColⅣ） mRNA expressions in GMC were analyzed by RT-PCR. The secretion of LN and Col lV in culture medium of rat＇s GMC was measured by radioimmunoassay. Results Ang-（1-7） significantly inhibited basal and TGF- β1-induced proliferation of cultured glomerular mesangial cells. Ang-（1-7） significantly down-regulated LN and ColⅣ mRNA expressions in glomerular mesangial cells and also inhibited the secretion of LN and Col IV induced by TGF-β1（ P 〈 0. 05 ）. Conclusions Ang-（ 1-7 ） can inhibit basal and TGF-β1-induced proliferation, and inhibit ECM secretion of cultured rat＇s glomerular mesangial ceils.