巨噬细胞移动抑制因子在大鼠实验性结肠炎肠组织中的表达

The expression of MIF in colon tissues of rats with TNBS-induced colitis

目的观察巨噬细胞移动抑制因子（MIF）在三硝基苯磺酸（TNBS）诱发的大鼠结肠炎肠组织中的表达及其与疾病严重程度的关系。方法采用TNBS／乙醇溶液灌肠制备大鼠结肠炎模型，24只动物分为正常组、轻、重模型组共3组。每天观察各组大鼠疾病活动指数（DAI）；生化法检测髓过氧化物酶（MPO）活性，采用逆转录，聚合酶链反应（RT-PCR）技术，对肠组织MIF的表达水平进行半定量测定。结果正常组DAI、MPO活性及MIFmRNA的表达水平分别为0．51±0．28、0．38±0．18、0．11±0．03；模型Ⅰ组5．04±0．73、1．68±0．45、0．65±0．04；模型Ⅱ组8．13±0．71、2．70±0．35、0．81±0．05。与正常组相比，模型组肠组织中MIFmR-NA的表达显著增强（P〈0．05），MPO活性及DAI也显著增高（P〈0．05），且在病情严重组增高尤为明显。结论大鼠结肠炎肠组织中MIF的表达升高，并与疾病严重程度正相关，提示其可能与大鼠结肠炎的发病有关。

Objective To observe the expression of macrophage migration inhibitory factor （MIF） in colon tissues of rats with 2,4, 6-trinitrobenzene sulfonic acid （ TNBS ） induced colitis and the relationship between MIF and the severity of the colitis. Methods 24 Sprague-Dawley rats were randomly divided into three groups ： normal group, model group I and model group Ⅱ. Rat colitis model was induced by 2,4,6-trinitrobenzene sulfonic acid （TNBS）, and disease activity index （DAI） was calculated every day. The myeloperoxidase （MPO） activity was detected by biochemistry, the expression of MIF in colon tissue was detected by semi-quantitative reverse transcriptional chain reactive （RT-PCR）. Results In normal group, DAI, MPO activity and MIF mRNA level were 0. 51±0. 28,0. 38±0. 18,0. 11±0.03, while in model group I were 5.04±0. 73,1.68±0.45,0.65±0.04 and in model group Ⅱ were 8. 13±0.71,2.70±0. 35,0.81±0.05. Compared with normal control, the expression of MIF mRNA significantly increased （ P 〈 0. 05 ） , MPO activity and DAI also significantly increased（ P 〈 0. 05 ）in model group, especially in model group ±. Condusion The expression of MIF increased in experimental colitis, and there was a positive relation between MIF expression and the severity of colitis, which suggested that MIF may be related to the pathogenesis of TNBS-induced colitis.