糖对稻曲病菌薄壁分生孢子萌发的影响

Effect of sugar on germination of thin-wall conidia of Ustilaginoidea virens

以琼脂平板为萌发基质,测定了10种糖化合物(木糖、果糖、山梨糖、核糖、甘露糖、葡萄糖、麦芽糖、半乳糖、乳糖和蔗糖)对稻曲病菌薄壁分生孢子萌发的影响。结果发现,在浓度为1%时,除蔗糖外,其余9种糖对孢子萌发均有不同程度的抑制作用,其中木糖、果糖、山梨糖和核糖的抑制作用强烈;孢子经木糖或果糖处理6h后,并未丧失活力;多种糖混合仍表现为抑制孢子萌发。当各种糖与起刺激作用的马铃薯煮汁分别混合时,表现出明显相反的两种效应,一是马铃薯汁的刺激作用可掩盖甘露糖、葡萄糖、麦芽糖、半乳糖、乳糖和蔗糖的抑制作用;二是木糖、果糖、山梨糖和核糖的抑制作用可掩盖马铃薯汁的刺激作用。表明外源糖化合物及某些生命活性物质可影响薄壁分生孢子萌发。

Effect of ten kinds of sugars on germination of thin-wall conidia of Ustilaginoidea virens （Cooke） Takahashi was determined with agar plate. The sugars were xylose, sorbose, ribose, fructose, mannose, galactose, maltose, glucose, lactose and sucrose. All the sugars exhibited inhibition to the conidia germination in varying degree at 1% concentration, except for sucrose. Inhibition of xylose, fructose, sorbose and ribose was very strong. The viability was not lost in the case that the conidia were treated with xylose or fructose for 6 h. Mixture of several sugars also showed inhibition to the conidia. Two completely different effects were exhibited from the mixture of each sugar and potato juice which was of stimulation to the conidia germination. One was that the inhibition of mannose, glucose, maltose, galactose, lactose and sucrose was covered up by the stimulation of the potato juice. The another was that the stimulation of the potato juice was covered up by the inhibition of xylose, fructose, sorbose and ribose. The results showed that germination of the thin-wall conidia could be controlled by external sugars and certain bioactive substances.