摘要
Periwinkle(Catharanthus roseus) yellows is a common disease in Hainan. Periwinkle′s leaf tissue with symptoms was assayed for phytoplasma infection by using PCR assay employing phytoplasma universal 16S rRNA gene primers (Rl6mF2/Rl6mR1). A PCR product (about 1.4 kb) was amplified from periwinkle showed yellows. Nucleotide sequencing and phylogenetic tree analysis showed that the amplified 16S rDNA contained 1 432 nucleotides, the most homology was 98.1% with the members of elm yellows group (16S r Ⅴ) and clustered in the same clade, while it was under 96.1% with other phytoplasma groups. Our results suggested that the phytoplasma sample belonged to 16S rⅤgroup and was tentatively named as Hainan periwinkle yellows phytoplasma (PY-Hn). This is the first report of existence of 16S r Ⅴ group phytoplasma in naturally infected periwinkle.
Periwinkle（Catharanthus roseus） yellows is a common disease in Hainan. Periwinkle′s leaf tissue with symptoms was assayed for phytoplasma infection by using PCR assay employing phytoplasma universal 16S rRNA gene primers （Rl6mF2/Rl6mR1）. A PCR product （about 1.4 kb） was amplified from periwinkle showed yellows. Nucleotide sequencing and phylogenetic tree analysis showed that the amplified 16S rDNA contained 1 432 nucleotides, the most homology was 98.1% with the members of elm yellows group （16S r Ⅴ） and clustered in the same clade, while it was under 96.1% with other phytoplasma groups. Our results suggested that the phytoplasma sample belonged to 16S rⅤgroup and was tentatively named as Hainan periwinkle yellows phytoplasma （PY-Hn）. This is the first report of existence of 16S r Ⅴ group phytoplasma in naturally infected periwinkle.
出处
《植物病理学报》
CAS
CSCD
北大核心
2009年第2期212-216,共5页
Acta Phytopathologica Sinica
基金
国家自然科学基金资助项目(30660103)
