联合检测6种肿瘤相关抗原抗体对卵巢癌的价值

Value of detecting the autoantibodies to six tumor-associated antigens in patients with ovarian cancer

目的评价survivin、p53、p16、cyclinB1、cyclinD1和cyclinE 6种肿瘤相关抗原(TAAs)抗体的联合检测对卵巢癌诊断及术后病情监测的价值。方法用间接酶联免疫吸附试验(ELISA)检测55例原发性上皮性卵巢癌和40例卵巢良性肿瘤,以及23例卵巢癌患者术后化疗期间的69份血清中以上6种TAAs自身免疫抗体,以流行病学方法分析结果,对比CA125分析联合检测6种TAAs抗体的价值。结果单独检测TAAs抗体诊断卵巢癌灵敏度在9.1%～23.6%,而6种TAAs联合并联试验诊断卵巢癌灵敏度为63.6%,特异度、阳性和阴性预测值及约登指数分别是85.7%、87.5%、60.0%及0.4935;联合检测6种TAAs抗体阳性率在卵巢癌不同组织学类型和临床分期间无统计学差异(P>0.05),且在卵巢癌术后化疗各组阳性率(8.7%～34.8%)较术前组(65.2%)均有明显降低(P<0.001);用此法诊断及监测卵巢癌与CA125比较无统计学差异(P>0.05)。结论联合检测血清中6种TAAs自身抗体能够提高卵巢癌的诊断质量,对卵巢癌诊断和术后病情监测可能具有重要参考价值。

Objective To evaluate the significance of detecting autoantibodies to tumor-associated antigens （TAAs） in diagnosis and post-operative monitoring of ovarian cancer, including survivin, p53, p16, cyclinB1, cyclinD1, and cyclinE. Methods The autoantibodies to the six TAAs were detected in serum of 55 patients with primary epithelial ovarian cancer and 40 patients with ovarian benign tumor, tients of ovarian cancer during chemotherapy after operation using enzyme and in 69 serum from 23 palinked immunosorbent assay （ELISA）. The clinical value of combining the six TAAs in ovarian cancer was compared with CA125. Results The sensitivity in diagnosing ovarian cancer using single antigen was 9. 1% -23.6%. When combining the six TAAs to detect the autoantibodies, it reached to 63. 6%, and the specificity, positive and negative predictive proportion, and kappa value were 85.7%, 87. 5%, 60. 0%, and 0. 4935. When combining the six TAAs, the positive rate of autoantibodies were not significantsy different among different histological types and clinic stages （P 〉0. 05）; in primary ovarian cancer patients during chemotherapy after operation, they （8. 7% - 34. 8% ） were significantly lower than in the same patients before operation （65. 2% ） （P 〈0. 001 ）. Conclusion Combining the six TAAs can increase the accuracy of the diagnosis of ovarian cancer.