用DNA杂交技术检测产β-内酰胺酶的临床分离菌株的TEM-1基因

TX Detection of TEM1 Fragment in Clinical Isolates of Positive βlactamase by DNA Blotting Hybridization

为了探求院内感染革兰氏阴性杆菌产生超广谱β内酰胺酶的基因决定机制，我们采用ＤＮＡ斑点杂交技术对所选临床分离的产β内酰胺的大肠埃希氏菌、克雷伯氏菌等进行了其质粒介导的β内酰胺酶基因类型分析。对２０株菌质粒用ＴＥＭ１探针检测的结果如下：１１株大肠埃希氏菌全与ＴＥＭ１探针杂交；６株肺炎克雷伯氏菌有３株与ＴＥＭ１探针杂交；１株已证明含有ＳＨＶ２超广谱β内酰胺酶的日勾维肠杆菌未与ＴＥＭ１探针杂交；２株不动杆菌有１株（溶血不动杆菌）与ＴＥＭ１探针杂交。

To detect the genic mechanism of ExtendedSpectrum βlactamase(Esbla)in clinical nosocomial gram negative isolates.DNA blotting hybridization was used to analyse plasmids of 20 isolates which produced βlactamase including E.Coli,K.Pneumoniae etc.The results show that 11 E.Coli are TEM1 probe positive;3 of 6 K.pneumoniae have TEM fragment;1 E.gergoviae which has been proved to have SHV2 Esbla was TEM1 negative and 2 acintobacter can't hybridize with TEM1 probe.