苯并(a)芘对肝细胞凋亡及脂质过氧化作用

Effect of BaP on Hepatocyte Apoptosis and Lipid Peroxidation

目的探讨苯并(a)芘(BaP)对小鼠肝细胞凋亡及其与脂质过氧化的关系。方法ICR小鼠灌胃染毒,染毒剂量分别为0、5、10、20、40mg/kg,应用吖啶橙/溴化乙啶(A0/EB)荧光双染法检测肝细胞凋亡情况并测定肝组织脂质过氧化主要终产物丙二醛(MDA)的含量。结果BaP各染毒剂量组肝细胞凋亡发生率与对照组比较,差异均有统计学意义(P<0.05),MDA含量40mg/kgBaP染毒剂量组与对照组比,较差异具有统计学意义(P<0.05)。结论BaP可诱发小鼠肝细胞发生凋亡和脂质过氧化,氧化损伤参与了介导细胞凋亡的过程。

[ Objective ] To study the relationship between BaP and hepatocyte apoptosis in mice and lipid peroxidation. [ Methods ] ICR mice were oral exposure with BaP for0, 5,10,20,40 mg/kg respectively. The major product of Lipid pemxidationMDA content in the liver was detected and the apoptosis were measured by AO/EB double fluorescence stain method. [Results]The apoptosis rate in BaP treated group were significantly higher than those in control group（ P 〈0.05）. In the comparison of the BaP treated group with MDA content of 40 mg/kg and the control group, the difference of apoptosis rate was significant（ P 〈0. 05）. [ Conclusion] BaP can induce apoptosis and lipid peroxidation in mice liver cells. Oxidative damage involves in the process of apoptosis.