冷暴露对大鼠血清中NO、NOS含量的影响

Effects of Cold Exposure on Serum NO and NOS Levels of Rats

目的通过检测大鼠血清中一氧化氮(NO)、一氧化氮合酶(NOS)含量的变化来探讨冷暴露对血管收缩功能的影响。方法将Wistar大鼠随机分为11个组(1个对照组,10个冷暴露组),在-30℃的冷舱中单笼放置30、60、80min,取出立即取血,-30℃80min复温4、8、16、24、72、120h采血,观察大鼠在冷暴露过程中及复温后血清中NO、NOS含量。结果NO的含量在冷暴露80min以前随时间延长其含量逐渐升高,与对照组比较,差异有统计学意义(P<0.05)。冷暴露后随着复温时间的延长,大鼠血清中NO的含量又逐渐降低,至复温120h时降至最低值,接近对照组水平。冷暴露30min,大鼠血清中NOS的浓度降低,与对照组比较,差异有统计学意义(P<0.05)。然后浓度逐渐上升,在复温4hNOS的浓度又降低,与对照组比较,差异有统计学意义(P<0.05)。在复温8hNOS的浓度显著升高,与对照组比较,差异有统计学意义(P<0.01)。然后NOS的浓度在波动中达到平衡,在复温120h时接近对照组水平。结论冷暴露的刺激会使大鼠血管舒缩功能发生改变,NO、NOS的含量也随之发生变化。

[ Objective ] To explore the effects of cold exposure on vasoconstriction through the measurement of the change in the levels of serum NO and NOS of rats. [ Methods] Wistar rats were divided into 11 groups randomly（ 1 control group, 10 groups for cold exposure）. They were individually placed into cold chamber of-30 ℃ for 30, 60, and 80 minutes and drew blood when they were taken out. Exposed others to cold condition at the temperature of -30 ℃ for 80min and drew blood after 4h, 8h, 16h, 24h, 72h, and 120h rewarming. The levels of serum NO and NOS of rats were observed during cold exposure and rewarming. [ Results] The level of NO went up as the length of time for cold exposure increased, indicating significant difference from the control group （ P 〈 0.05）. After the cold exposure of 80 minutes, the level of NO decreased as the length of rewarming increased, reaching the bottom as the rewarming lasted for 120h which was close to the level of control group. The level of NOS went clown in the 30 minutes cold exposure, showing the significant difference （ P 〈 0.05 ） compared with the control group. Then the level of NOS increased, and decreased in the 4h rewarming, showing significant difference （ P 〈 0.05 ） compared with the control group. After rewarming for 8 hours, the level of NOS significantly increased, showing significant difference （ P 〈 0.01 ） compared with the control group. Then the level of NOS reached its balance in fluctuations and was close to that of the control group after the 120h rewarming. [ Conclusion] Cold exposure will change the vasoconstriction of the rats and the level of NO, NOS.