葡萄籽提取物抑制乳腺癌细胞生长的实验研究

Experimental study on the role of grape seed extract in inhibiting the growth of breast cancer cells

目的:探讨葡萄籽提取物(grape seed extact,GSE)抑制乳腺癌细胞生长的分子机制。方法:利用四甲基偶氮唑蓝(MTT)法检测GSE对乳腺癌细胞的抑制作用,并筛选合适的GSE实验浓度;流式细胞术检测GSE对乳腺癌细胞内活性氧(ROS)产生的影响;半定量RT-PCR法检测肿瘤细胞中肿瘤相关基因Bcl-2和Bax表达的水平。结果:GSE抑制乳腺癌细胞的生长,作用具有剂量依赖性(P<0.01),IC50为100μg/ml;GSE降低肿瘤细胞内ROS的产生;GSE能减少乳腺癌细胞中Bcl-2基因的表达,对Bax基因无影响,其Bcl-2/Bax值显著降低(P<0.01)。结论:GSE抑制乳腺癌细胞生长,可能是通过降低ROS的产生,减少相关基因Bcl-2的表达水平,进而降低Bcl-2/Bax值而实现的。

Objective：To investigate the role of grape seed extract （GSE） in inhibiting the growth of human breast cancer cells. Methods：MTT assay was employed to determine the inhibitory effect of GSE on human breast cancer cells, and suitable experimental concentration of GSE was screened. The influence of GSE on the generation of reactive oxygen species （ROS） in breast cancer cells was evaluated by flow eytometry;semiquantitative RT-PCR was used to detect the expression level of Bcl-2 gene and Bax gene related to tumor apoptosis. Results： GSE inhibited the growth of breast cancer cells in a dose-dependent manner（P 〈 0.01 ）, and the proper dosage of IC_50 was 100 μg/ml. GSE might reduce the generation of ROS as well as the expression of Bcl-2 gene in tumor cells, but had no influence on the expression of Bax gene, and the value of Bcl-2/Bax decreased significantly （ P 〈 0. 01 ）. Conclusions ： GSE can inhibit the growth of human breast cancer cells, which might be achieved by reducing the generation level of ROS and regulating the expression of Bcl-2 gene and Bax gene.