摘要
目的:采用高效液相色谱法研究并建立苦参药材生物碱成分的HPLC指纹图谱,同时进行定性和定量相似度评价。方法:采用Lichrospher ODS-2色谱柱(250mm×4.6mm,5μm),甲醇-乙腈(1∶1)为有机相,0.1%氨水为水相,梯度洗脱,流速1.0mL/min,检测波长210nm,测定苦参药材的HPLC指纹图谱,统计分析确定共有模式;以最接近共有模式的苦参药材为特征对照,采用夹角余弦法(Cosθ)和相对欧氏距离法(RED)对不同产地苦参药材的HPLC指纹图谱进行定性与定量相似度评价。结果:苦参高效液相色谱指纹图谱中,以氧化苦参碱为参照峰,确定了10个特征峰。相似度比较结果显示,不同产地药材样品夹角余弦都大于0.9,表明各产地苦参药材化学组成一致性较好,相对欧氏距离由0.1至0.4不等,可见药材中特征成分含量上存在差异。结论:该方法重复性好,可为科学评价及有效控制苦参药材的质量提供参考。
Aim: To establish a sensitive and specific HPLC method for the systematic evaluation of qualitative and quantitative similarities of alkaloid components of Sophora flavescens Ait. from different origins. Methods: Alkaloid components of Sophora flavescens were separated on a Lichrospher ODS-2 ( 250 mm×4.6mm,5μm) column with a linear gradient elution model using a mobile phase consisting of methanol-acetonitrile (1: 1) and 0. 1% aqueous ammonia at a flow rate of 1.0 mL/min. The detection wavelength was set at 210 nm and the column temperature 30℃. The similarities of the corresponding HPLC fingerprints were evaluated by cosine ( Cos O) of vectors and relative Euclidean distance methods, in terms of the chemical constituents and the con- tents, respectively. Results: Ten common peaks were confirmed in the HPLC fingerprints of Sophora flavescens using oxymatrine as the reference peak. Similarity analyses indicated that no significant difference between differ- ent origins was found in the qualitative similarity although obvious quantitative similarity differences were observed. Conclusion: HPLC fingerprints show good repeatability. It can be used as a means to assess and control the quality of Sophora flavescens.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2009年第2期139-143,共5页
Journal of China Pharmaceutical University