复方莪术油溶液抗H5N1亚型禽流感病毒的作用研究

Antiviral effect on H5N1 avian influenza virus of compound of Curcumazedoaria volatile oil and Hypericin perforatum extract liquid

目的:对复方莪术油溶液在流感病毒的MDCK细胞上和在非免疫鸡内抗禽流感H5N1亚型病毒的作用进行了研究。方法:采用细胞病变效应(CPE)抑制试验和MTT测定法,研究复方莪术油溶液及其有效成分莪术醇和金丝桃素在MDCK细胞上对H5N1病毒的灭活作用,对病毒感染的阻滞作用,对病毒吸附的抑制作用以及对被病毒入侵的细胞的治疗作用;另外,用复方莪术油溶液对14日龄非免疫试验鸡连续经口喂药1周,采用禽流感病毒H5N1攻击鸡群,攻毒的同时开始给药,观察药物对鸡的保护作用。同时与单方莪术油溶液、贯叶连翘水提取液和阳性对照药奥司他韦以及金刚烷胺进行了比较。结果:在体外实验中,复方莪术油溶液及其有效成分莪术醇和金丝桃素通过灭活病毒,阻滞病毒感染,抑制病毒吸附和治疗病毒引起的损伤而发挥抗H5N1亚型禽流感病毒的作用;在体内实验中它们对染毒鸡具有较好的预防保护作用,可延长染毒鸡的平均存活天数,与病毒对照组比较具有显著性差异(P<0.01),奥司他韦与金刚烷也具有相似的抑制病毒的作用。结论:复方给药的效果优于单方,并且主要在预防病毒感染环节发挥抑制作用。

Aim： To investigate the antiviral effect of compound of Curcuma zedoaria volatile oil and Hypericin perforatum extract liquid on H5N1 avian influenza virus （AIV） in MDCK cell line and non-AIV-immunized chickens. Methods： CPE inhibiton and MTT assay were used to study the inhibition of compound of Curcuma zedoaria volatile oil and Hypericin perforatum extract liquid, cureumenol and hypericin on H5N1 in MDCK, the retarding effect on H5N1 infection, the inhibition on the virus adsorption, and the therapeutic effect on the virusinfected cells. In addition, after one-week oral feeding of the liquid, non-AIV-immunized chickens were exposed to H5N1 AIV and the protecting effects of the liquid on the chickens were evaluated. The effect was also assessed after the chickens were simultaneously exposed to the liquid and and the attacking H5N1. The antiviral effects of oseltamivir and amantadine were also detected as the control groups. Results： In vitro results showed that compound of Curcuma zedoaria volatile oil and Hypericin pe（foratum extract liquid was effective via the mechanisms including virus killing, virus inhibiting, the prevention of the virus spread, and the treatment of the virus-related injury in MDCK. In vivo studies indicated that compound of Curcuma zedoaria volatile oil and Hypericin perfora- turn extract liquid prolonged the mean survival days of the chickens, showing significant preventive effect in the chickens. It was also proved that compound of Curcuma zedoaria volatile oil and Hypericin pe（foratum extract liq- uid was more effective than zedoary oil and Hypericum perforatum extract. Conclusion： Compound of Curcuma zedoaria volatile oil and Hypericin perforatum extract liquid is superior to zedoary oil and Hypericum perforatum in the treatment of H5N1 via the prevention of the virus infection and it might be very promising in the development into into effective anti-H5N1 virus drug.