吉西他滨抑制骨肉瘤细胞系MG-63增殖和诱导凋亡的实验研究

Gemcitabine inhibits cell growth and induces apoptosis of osteosarcoma cell line MG-63

目的研究吉西他滨对骨肉瘤细胞系MG-63的增殖抑制和凋亡诱导作用。方法利用光镜检测吉西他滨作用后MG-63的形态学变化;MTT法检测吉西他滨对MG-63的增殖抑制;流式细胞仪AnnexinV-FITC法检测细胞凋亡,琼脂糖凝胶电泳检测DNA ladder。结果吉西他滨可明显地抑制MG-63细胞生长,各实验组24、48、72小时抑制率随时间的增加而增加,差异有统计学意义（P〈0.05）,0.1 mg/L及以上浓度的吉西他滨对MG-63骨肉瘤细胞的抑制率可达50%,0.1-100 mg/L的吉西他滨对MG-63的抑制率相似。吉西他滨可诱导MG-63细胞凋亡,在不同浓度吉西他滨（0.1,1.0,10.0,100 mg/L）作用48小时后,MG-63的凋亡率差异无统计学意义（P〉0.05）。并且经琼脂糖凝胶电泳可以显示DNA ladder。结论吉西他滨对骨肉瘤细胞系MG-63具有增殖抑制作用,并且各实验组24、48、72小时抑制率随时间的增加而增加;吉西他滨对骨肉瘤细胞系MG-63具有诱导凋亡作用;0.1-100 mg/L吉西他滨对MG-63的增殖抑制和诱导凋亡作用相似。

Objective To investigate the inhibitory effect of gemcitabine on cell growth and the inductive effect on apoptosis of osteosarcoma cell line MG-63. Methods The cultured MG-63 cells were treated with gemcitabine. The morphological changes of MG-63 cells were examined by phase contrast microscope ;cell growth rates were assessed by MTT bromide colorimetric assay. The apoptosis of MG-63 cells was measured by flow cytometry （FCM） with Annexin-V-FITC assay and DNA ladder by agarose electrophoresis. Results Gemcitabine significantly inhibited cell growth and the inhibitory effect was in a time-dependent manner evidenced by MTT assay and morphological changes. The apoptotic index measured by FCM increased correspondingly with the concentration of gemcitabine and the reaction time. Treatment of MG-63 with different concentrations of gemcitabine （0, 0. 1,1. 0, 10. 0,100 mg/L） for 48 h, the apoptotic index was not significantly different （P〉 0. 05）. DNA ladder was demonstrated on DNA electrophoresis. Conclusion Gemcitabine can significantly inhibit MG-63 cell growth in a time-dependent manner,and it can also induce the apoptosis of MG-63 cells.