鼻咽癌染色体端粒联合的研究

Study on Telomeric Association in Nasopharyngeal Carcinoma

采用Ｇ带技术，对２５例鼻咽癌患者外周血和鼻咽癌细胞株ＣＮＥ的染色体端粒联合现象进行观察。结果表明：鼻咽癌细胞端粒联合率（３５．０７％）显著高于对照组（２０．２７％）（Ｐ＜０．０１），在两组中，端粒联合在各号染色体上的分布基本一致（Ｐ＞０．０５）。鼻咽癌组的１、２、３、４、７、１１、１５、１７号染色体端粒联合率明显高于对照组（Ｐ＜０．０５），在细胞株ＣＮＥ中，９４％的细胞分裂相中显示了端粒联合，其中２、３、５、１１、１６号染色体发生端粒联合的频率较高。实验中还发现，端粒联合主要以染色单体型为主，染色体型较为少见。

The chromosomal telomeric association in peripheral blood lymphocytes from 25 untreated patients with nasopharyngeal carcinoma (NPC) and in NPC cell lines (CNE) was investigated by usig chromosome G banding technique. The cell telomeric association rate of patients (35.07%) was found to be higher than that of controls(20.27%) (P<0.01),while in the two groups the distributions of chromosomes involved were approximately the same (P>005). The telomeric association rates of patients were significantly higher than those of control in chromosomes 1,2,3,4,7,11,15,and 17(P<0.05). In NPC lines (CNE), 94% of the cell division phases showed telomeric association. Chromosomes 2,3,5,11 and 16 had higher frequencies of association than other chromosomes. The most frequent telomeric association was single chromatid association.