CD44分子在葡萄膜与结膜黑色素瘤中的表达及其意义

Expression of CD44 Molecule and Its Significance in Uveal and Conjunctival Melanomas

为评价ＣＤ４４分子过量表达在葡萄膜与结膜黑色素瘤发生发展过程中的作用，应用流式细胞术（ＦＣＭ）及细胞免疫荧光染色技术对葡萄膜恶性黑色素瘤（ＵＭＭ）、结膜色素痣（ＣＮ）及恶性黑色素瘤（ＣＭＭ）的ＣＤ４４Ｖ表达进行定量检测。结果表明：７例ＣＭＭ较５例ＣＮ的ＣＤ４４Ｖ表达量明显增加（Ｐ＜０．０５），７例ＣＭＭ及４０例ＵＭＭ的ＣＤ４４Ｖ表达阳性率分别为７１．４３％及６２．５％；ＵＭＭ中伴巩膜受侵犯者较不伴巩膜受侵犯者的ＣＤ４４Ｖ表达量明显增加（Ｐ＝０．０１０５）；ＵＭＭ的ＣＤ４４Ｖ表达量与增殖指数（ＰＩ）、Ｓ期细胞百分率（ＳＰＦ）呈显著负相关（Ｐ＜０．０１；Ｐ＜０．０５）。以上表明ＣＤ４４Ｖ过量表达与ＣＭＭ及ＵＭＭ的发生有关，并与ＵＭＭ局部浸润有关。

The aim of this study was to evaluate the role of over expression of CD44 molecule in the development and progression of uveal and conjunctival melanomas. Flow cytometry (FCM) and immunofluorescence methods were used for detecting the CD44V expression in uveal malignant melanomas (UMM), conjunctival nevi (CN) and conjunctival malignant melanomas(CMM). The expression content of CD44 in 7 cases of CMM was significantly higher than that in 5 cases of CN (P<005);the CD44V positive expression percentages in 7 cases of CMM and 40 cases of UMM were 71.43% and 62.50% respectively; the expression content of CD44V in UMM was related to scleral invasion (P=0.0105); there was a negative correlation between the expression content of CD44V and proliferative index (PI), Sphase fraction (SPF) (P<0.01;P<0.05).The results suggested that the over expression of CD44V might be involved in the development of CMM and UMM and related to local infiltration ability of UMM and that the CD44V expression content detected by FCM might be helpful in discriminating CN From CMM, but this waited for further research confirmation.