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猪CFL2b基因cDNA克隆初步分析 被引量：4

Cloning and Sequence Analysis of cDNA Encoding CFL2b from Porcine
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摘要 利用基因表达谱芯片分析法筛选出与大白猪高肌肉产量-肌纤维形成有关的CFL2b基因.参考人和小鼠CFL2b基因序列,采用SMART-RACE技术结合EST序列拼接技术,从猪骨骼肌肌肉中首次克隆了猪CFL2b的全长cDNA序列(GenBank登录号EU561660,EU561661),Northern杂交检测CFL2b基因mRNA.结果表明,猪CFL2b基因含有2个转录本,长转录本3012bp,短转录本1466bp.CFL2b基因在多种真核生物中都有表达,且编码区序列非常保守,开放式读码框501bp编码了166个氨基酸的蛋白质.氨基酸序列分析表明,猪CFL2b基因与人和小鼠氨基酸同源性分别为100%和99.1%.核苷酸序列相似性分别为88.1%和74.9%.
作者 赵微 曾瑞霞 巴彩凤 宋慧娟 苏玉虹
机构地区 辽宁医学院实验动物教研室 辽宁医学院高校分子细胞生物学与新药开发重点实验室 辽宁医学院畜牧兽医学院
出处 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2009年第4期388-392,共5页 Chinese Journal of Biochemistry and Molecular Biology
基金 辽宁省自然科学基金(No.20072205) 辽宁省科技厅基金(No.2007408001-7)~~
关键词 猪 CFL2b CDNA SMART RACE 肌纤维性状 序列分析 NORTHERN杂交
分类号 Q78 [生物学—分子生物学]
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参考文献15

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二级参考文献46

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共引文献24

同被引文献34

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引证文献4

二级引证文献7

中国生物化学与分子生物学报
2009年 第4期

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