黄体功能不全患者分泌期子宫内膜孕激素受体的半定量研究

Semiquantitative Measurement of Progesterone Receptors in Lutealphasedefect Endometrial Cells During Secretory Phase

为进一步探讨黄体功能不全（ＬＰＤ）的病因，采用免疫组织化学法对７名ＬＰＤ患者分泌期子宫内膜上的孕激素受体（ＰＲ）进行了半定量测定。结果显示：ＰＲ普遍存在于子宫内膜腺上皮细胞和间质细胞的胞浆及胞核中。在ＬＰＤ患者与正常子宫内膜者血清孕酮值无显著性差异的情况下，ＬＰＤ患者的子宫内膜腺上皮细胞胞核的ＰＲ半定量评分（６．７５±２．５７分）明显低于正常内膜者（９．５０±１．６４分），Ｐ＜０．０５。提示：ＬＰＤ患者分泌早－中期宫内膜腺上皮细胞胞核ＰＲ含量的下降，降低了子宫内膜对正常的孕酮刺激产生的反应，从而导致分泌机能不足。

To investigate the changes of endometrial progesterone receptor (PR) of lutealphasedefect(LPD) patients during the secretory phase,thirteen patients with complaints of infertility or habitual abortion were studied.During the earlymid secretory phase,endometrial tissue was obtained by dilatation and curettage (D & C) for histological and receptor study;meanwhile serum E2,P,FSH,LH and PRL were measured.Based on histologic diagnosis,the patients were divided into two groups:the LPD group (n=7) and the normal control group(n=6).PR content was determined by immunohistochemical (IHC) assay.The results showed that during the earlymid luteal phase a significantly low PR content on endometrial glandular nucleus was observed in LPD group,compared with norml control(6.75±2.57 vs 9.50±1.64 P<0.05),but no difference in serum progesterone was noted between the two groups.These findings suggest that during earlymid secretory phase,PR content on endometrial glandular nucleus decreases in LPD cases,which results in deficient response of endometrium to proper stimulus of progesterone.This change may cause endometrial secretory deficiency and blockade of embreyo implantation.That is why infertility or habitual abortion happened.