脂蛋白脂酶基因HindⅢ酶切多态性与维吾尔族2型糖尿病及体内脂质谱的关联研究

Association of Polymorphism of Lipoprotein Lipase Gene with Type 2 Diabetes and the Lipids Spectrum in Uygurs

目的探讨脂蛋白脂酶(LPL)基因HindⅢ酶切多态性与维吾尔族2型糖尿病及其体内脂质谱的关系。方法依据病例-同胞对照设计方法,采用全自动生化分析仪等技术测定维吾尔族人群体内脂质谱;应用聚合酶链反应-限制性内切酶长度多态性(PCR-RFLP)方法,对维吾尔族62例2型糖尿病患者、62例糖耐量受损者和124名正常人LPL基因HindⅢ酶切多态性进行分析。结果三组人群的LPL基因的基因型分布和等位基因频率均无统计学意义。H+H+组、H-H-组、H+H-组的TG水平分别为(2.26±1.35)、(1.73±1.31)、(1.80±0.95)mmol/L,三种基因型与体内脂质等指标比较,突变型H+H+组的TG明显大于其他各组。多因素Logistic回归分析显示,TG(P=0.034)和腰围(P=0.001)是2型糖尿病独立危险因素。结论LPL基因HindⅢ酶切多态性与新疆维吾尔族人群2型糖尿病的危险性并无统计学上的相关性,LPL基因突变可能是引起血浆TG水平升高的因素之一。

Objective To study the association of LPL gene polymorphism with type 2 diabetes and the lipids spectrum in Uygurs. Methods Based on the case-siblings control design, the lipid spectrum of Uygur were tested by Automatic biochemical analyzer and the polymorphism of LPL gene were analyzed by RLFP with Hind Ⅲ in 62 T2DM patients, 62 IGT patients and 124 normal controls of Uygurs. Results The genotype distribution and allele frequencies of LPL gene in three groups were not statistically significant. The average of TG in H＋H＋group, H-H-group and H＋H-group were 2.26, 1.73 and 1.80 mmol/L; Compared with the three genotypes and lipid indicators, TG content of Mutant H ＋H ＋ group were higher than that in other groups. Multi-factor Logistic regression analysis showed T2DM was closely related to TG （P=0.034）and waist circumference （P=-0.001）. Conclusion The relation between LPL gene polymorphism by Hind Ⅲ and the risk for T2DM in Xinjiang Uygur population are no statistical relevance, LPL gene mutations may be one of the factors causing elevated levels of plasma TG.