成骨生长肽羧基端片段衍生物H86A对去卵巢大鼠骨计量学的影响

Effects of OGP derivate H86A on Bone Metrology in Ovariectomized Rats

目的探讨成骨生长肽羧基端片段及其衍生物H86A对去卵巢(OVX)骨质疏松大鼠骨计量学的影响。方法将32只3月龄SD雌性大鼠分为4组,1～3组行OVX手术,1、2组术后分别给予高低剂量的H86A,3组给予安慰剂,4组为假手术组(SHAM)。12周后处死动物,分离左侧胫骨,制成不脱钙骨切片,检测并分析骨计量学有关指标。结果H86A高剂量组(H86AH)与去卵巢给安慰剂组(OVX)比较,OV/BV减少(P<0.01),其他指标无统计意义;与SHAM组比较,BV/TV、Tb.Th、Tb.N减少(P<0.01),Tb.Sp、OV/BV增加(P<0.01),其他指标无统计学意义(P>0.05)。H86A低剂量(H86AL)组与OVX组比较,BV/TV增加(P<0.05),OV/BV减少(P<0.01),sLS/BS、Ms/BS增加(P<0.01),与SHAM组比较,BV/TV、Tb.Th、Tb.N减少(P<0.01),Tb.Sp、OV/BV增加(P<0.01),sLS/BS、MS/BS增加(P<0.01),其他指标无统计学意义。结论成骨生长肽羧基端片段及其衍生物可以提高骨量,改善骨组织的微结构,改善骨质疏松的骨质量。

Objective To investigate the effects of OGP （10-14） and its derivate H86A on bone metrology in rats with ovarieetomy-reduced osteoporosis. Methods Thirty-two female Sprague-Dawley rats, three months old, were randomly divided into 4 groups. Rats in groups 1 to 3 were bilaterally ovarieetomized（OVX） and the others in group 4 were sham-operated. After the operation, rats in group 1 and group 2 were received derivate of OGP（10-14） H86A daily at different concentration. Group 3 were given placebo. Rats were killed after 12 weeks. Left tibias were disconnected and made into non-decalcified bone slice, and then they were detected and analyzed on indexes which were relative with bone metrology. Results Compared with Group 3, no other indexes except OV/BV was obviously different in rats with high dose H86A （P〈0.01）. Compared with group 4, BV/TV, Tb.Th and Tb.N were diminished （P〈0.01）, Tb.Sp and OV/BV were raised （P〈0.01） in rats with high dose H86A, but no statistically significant in other indexes. Compared with rats in group 3, rats with low dose H86A had increased BV/TV （P〈0.05）, sLS/BS, Ms/BS （P〈0.01）, and decreased OV/BV （P〈0.01）; but BV/ TV, Tb.Th, Th.N were decreased （P〈0.01） and Tb.Sp, OV/BV, sLS/BS, MS/BS were increased compared to rats in SHAM. Conclusion OGP （10-14） and its derivate can increase bone mass, improve microstruetural of bone, and enhance bone quality of rats with osteoporosis.